Systematic toxicological analysis of drugs and poisons in biosamples by hyphenated chromatographic and spectroscopic techniques

被引:43
作者
Polettini, A [1 ]
机构
[1] Univ Pavia, Dept Legal Med & Publ Hlth, I-27100 Pavia, Italy
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1999年 / 733卷 / 1-2期
关键词
systematic toxicological analysis; drug screening; reviews; detection; spectroscopic;
D O I
10.1016/S0378-4347(99)00264-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The introduction of hyphenated chromatographic-spectroscopic techniques represented a substantial step-forward for Systematic Toxicological Analysis (STA), increasing the amount and quality of information obtainable from the analysis of a biological sample, and enhancing the possibilities of identifying unknown drugs and poisons. STA methods based either on GC-MS or on HPLC-UV published in the last decade are reviewed in this paper. The different analytical phases, i.e. sample preparation (pretreatment, extraction, derivatisation), chromatographic separation and detection/identification are examined in detail in order to emphasise the complementarity of the two approaches, In addition, the first STA method based on HPLC-MS is illustrated and some applications of TLC-UV to drug screening are also described, Finally, an overview of semi- and fully-automated STA methods is given. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:47 / 63
页数:17
相关论文
共 95 条
  • [81] PRAGST F, 1997, UV SPECTRA TOXIC COM
  • [82] Rasanen I, 1996, HRC-J HIGH RES CHROM, V19, P313
  • [83] RUSSELL S, TIAFTNET ON LINE REV
  • [84] Sadeg N, 1997, CLIN CHEM, V43, P498
  • [85] Derivatization procedures for gas chromatographic mass spectrometric determination of xenobiotics in biological samples, with special attention to drugs of abuse and doping agents
    Segura, J
    Ventura, R
    Jurado, C
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1998, 713 (01): : 61 - 90
  • [86] AUTOMATED IDENTIFICATION BY COMPUTER OF THE MASS-SPECTRA OF DRUGS IN URINE OR SERUM EXTRACTS
    SMITH, NB
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 1994, 18 (01) : 16 - 21
  • [87] PROBABILITY-BASED-MATCHING ALGORITHM WITH FORWARD SEARCHING CAPABILITIES FOR MATCHING UNKNOWN MASS-SPECTRA OF MIXTURES
    STAUFFER, DB
    MCLAFFERTY, FW
    ELLIS, RD
    PETERSON, DW
    [J]. ANALYTICAL CHEMISTRY, 1985, 57 (06) : 1056 - 1060
  • [88] Traqui A, 1994, J FORENSIC SCI, V50, P254
  • [89] TURCANT A, 1991, CLIN CHEM, V37, P1210
  • [90] POSTMORTEM TISSUE SAMPLES - AN ALTERNATIVE TO URINE AND BLOOD FOR DRUG ANALYSIS IN RACEHORSES
    UBOH, CE
    RUDY, JA
    RAILING, FA
    ENRIGHT, JM
    SHOEMAKER, JM
    KAHLER, MC
    SHELLENBERGER, JM
    KEMECSEI, Z
    DAS, DN
    SOMA, LR
    LEONARD, JM
    [J]. JOURNAL OF ANALYTICAL TOXICOLOGY, 1995, 19 (05) : 307 - 315