Highly efficient peptide separations in proteomics Part 2: Bi- and multidimensional liquid-based separation techniques

被引：81

作者：

Sandra, Koen ^{[1
,2
]}

Moshir, Mahan ^{[2
]}

D'hondt, Filip ^{[2
]}

Tuytten, Robin ^{[2
]}

Verleysen, Katleen ^{[2
]}

Kas, Koen ^{[2
]}

Francois, Isabelle ^{[3
]}

Sandra, Pat ^{[1
,3
]}

机构：

[1] RIC, B-8500 Kortrijk, Belgium

[2] VIB Bio Incubator, B-9052 Zwijnaarde Ghent, Belgium

[3] Univ Ghent, Dept Organ Chem, B-9000 Ghent, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 11-12期

关键词：

Proteomics; Peptides; Multidimensional; Chromatography; Electrophoresis; On-line; Off-line; TANDEM MASS-SPECTROMETRY; HYDROPHILIC-INTERACTION CHROMATOGRAPHY; PROTEIN IDENTIFICATION TECHNOLOGY; CATION-EXCHANGE CHROMATOGRAPHY; LARGE-SCALE IDENTIFICATION; REVERSED-PHASE HPLC; PEAK-CAPACITY; ION-EXCHANGE; OFF-LINE; 2-DIMENSIONAL ELECTROPHORESIS;

D O I：

10.1016/j.jchromb.2009.02.050

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multidimensional liquid-based separation techniques are described for maximizing the resolution of the enormous number of peptides generated upon tryptic digestion of proteomes, and hence, reduce the spatial and temporal complexity of the sample to a level that allows successful mass spectrometric analysis. This review complements the previous contribution on unidimensional high performance liquid chromatography (HPLC). Both chromatography and electrophoresis will be discussed albeit with reversed-phase HPLC (RPLC) as the final separation dimension prior to MS analysis. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1019 / 1039

页数：21

共 147 条

[11] Assessing the dynamic range and peak capacity of nanoflow LC-FAIMS-MS on an ion trap mass spectrometer for proteomics [J].

Canterbury, Jesse D. ;

Yi, Xianhua ;

Hoopmann, Michael R. ;

MacCoss, Michael J. .

ANALYTICAL CHEMISTRY, 2008, 80 (18) :6888-6897

[12] Capillary isoelectric focusing-based multidimensional concentration/separation platform for proteome analysis [J].

Chen, JZ ;

Balgley, BM ;

DeVoe, DL ;

Lee, CS .

ANALYTICAL CHEMISTRY, 2003, 75 (13) :3145-3152

[13]

Chen JZ, 2002, ELECTROPHORESIS, V23, P3143, DOI 10.1002/1522-2683(200209)23:18<3143::AID-ELPS3143>3.0.CO

[14]

2-7

[15] Proteomic analysis with integrated multiple dimensional liquid chromatography/mass spectrometry based on elution of ion exchange column using pH steps [J].

Dai, J ;

Shieh, CH ;

Sheng, QH ;

Zhou, H ;

Zeng, R .

ANALYTICAL CHEMISTRY, 2005, 77 (18) :5793-5799

[16] Protein phosphorylation and expression profiling by Yin-Yang multidimensional liquid chromatography (Yin-Yang MDLC) mass spectrometry [J].

Dai, Jie ;

Jin, Wen-Hai ;

Sheng, Quan-Hu ;

Shieh, Chia-Hui ;

Wu, Jia-Rui ;

Zeng, Rong .

JOURNAL OF PROTEOME RESEARCH, 2007, 6 (01) :250-262

[17]

DAVIS JM, 1983, ANAL CHEM, V55, P418, DOI 10.1021/ac00254a003

[18] Automated LC-LC-MS-MS platform using binary ion-exchange and gradient reversed-phase chromatography for improved proteomic analyses [J].

Davis, MT ;

Beierle, J ;

Bures, ET ;

McGinley, MD ;

Mort, J ;

Robinson, JH ;

Spahr, CS ;

Yu, W ;

Luethy, R ;

Patterson, SD .

JOURNAL OF CHROMATOGRAPHY B, 2001, 752 (02) :281-291

[19] Two-dimensional reversed-phase x ion-pair reversed-phase HPLC: An alternative approach to high-resolution peptide separation for shotgun proteome analysis [J].

Delmotte, Nathanael ;

Lasaosa, Maria ;

Tholey, Andreas ;

Heinzle, Ehnar ;

Huber, Christian G. .

JOURNAL OF PROTEOME RESEARCH, 2007, 6 (11) :4363-4373

[20] Comprehensive multi-dimensional liquid chromatographic separation in biomedical and pharmaceutical analysis: a review [J].

Dixon, Steven P. ;

Pitfield, Ian D. ;

Perrett, David .

BIOMEDICAL CHROMATOGRAPHY, 2006, 20 (6-7) :508-529

← 1 2 3 4 5 6 7 8 9 10 →