αB-crystallin extracellularly suppresses ADP-induced granule secretion from human platelets

被引：14

作者：

Enomoto, Yukiko ^{[2
]}

Adachi, Seiji ^{[1
]}

Matsushima-Nishiwaki, Rie ^{[1
]}

Niwa, Masayuki ^{[3
]}

Tokuda, Haruhiko ^{[4
]}

Akamatsu, Shigeru ^{[5
]}

Doi, Tomoaki ^{[6
]}

Kato, Hisaaki ^{[6
]}

Yoshimura, Shinichi ^{[2
]}

Ogura, Shinji ^{[6
]}

Iwama, Toru ^{[2
]}

Kozawa, Osamu ^{[1
]}

机构：

[1] Gifu Univ, Dept Pharmacol, Grad Sch Med, Gifu, Japan

[2] Gifu Univ, Dept Neurosurg, Grad Sch Med, Gifu, Japan

[3] Gifu Univ, Div Med Sci, United Grad Sch Drug Discovery & Med Informat Sci, Gifu, Japan

[4] Natl Hosp Geriatr Med, Dept Clin Lab, Natl Ctr Geriatr & Gerontol, Aichi, Japan

[5] Matsunami Gen Hosp, Dept Intens Care Med, Gifu, Japan

[6] Gifu Univ, Dept Emergency & Disaster Med, Grad Sch Med, Gifu, Japan

来源：

FEBS LETTERS | 2009年 / 583卷 / 15期

关键词：

Heat shock protein; alpha B-crystallin; Platelet; Adenosine diphosphate; Platelet-derived growth factor-AB; Serotonin; HEAT-SHOCK PROTEINS; STRESS-PROTEINS; HSP27; PHOSPHORYLATION; RECEPTOR; RELEASE;

D O I：

10.1016/j.febslet.2009.06.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

alpha B-crystallin, a low-molecular-weight heat shock protein (HSP), has binding sites on platelets. However, the exact role of alpha B-crystallin is not clarified. In this study, we investigated the effect of alpha B-crystallin on platelet granule secretion. alpha B-crystallin attenuated the adenosine diphosphate (ADP)-induced phosphorylation of p44/p42 mitogen-activated protein kinase (MAPK) and p38 MAPK. The ADP-stimulated HSP27 phosphorylation was markedly reduced by alpha B-crystallin. alpha B-crystallin significantly suppressed the ADP- induced secretions of both platelet-derived growth factor (PDGF)-AB and serotonin. Therefore, our results strongly suggest that alpha B-crystallin extracellularly suppresses platelet granule secretion by inhibition of HSP27 phosphorylation via p44/p42 MAPK and p38 MAPK. (c) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2464 / 2468

页数：5

共 29 条

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