RecA dimers serve as a functional unit for assembly of active nucleoprotein filaments

被引：11

作者：

Forget, Anthony L. ^{[1
]}

Kudron, Michelle M. ^{[1
]}

McGrew, Dharia A. ^{[1
]}

Calmann, Melissa A. ^{[1
]}

Schiffer, Celia A. ^{[1
]}

Knight, Kendall L. ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA 01605 USA

来源：

BIOCHEMISTRY | 2006年 / 45卷 / 45期

关键词：

D O I：

10.1021/bi060938q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

All RecA-like recombinase enzymes catalyze DNA strand exchange as elongated filaments on DNA. Despite numerous biochemical and structural studies of RecA and the related Rad51 and RadA proteins, the unit oligomer(s) responsible for nucleoprotein filament assembly and coordinated filament activity remains undefined. We have created a RecA fused dimer protein and show that it maintains in vivo DNA repair and LexA co-protease activities, as well as in Vitro ATPase and DNA strand exchange activities. Our results support the idea that dimeric RecA is an important functional unit both for assembly of nucleoprotein filaments and for their coordinated activity during the catalysis of homologous recombination.

引用

页码：13537 / 13542

页数：6

共 42 条

[21] ATP-mediated changes in cross-subunit interactions in the RecA protein [J].

Logan, KM ;

Forget, AL ;

Verderese, JP ;

Knight, KL .

BIOCHEMISTRY, 2001, 40 (38) :11382-11389

[22] The bacterial REcA protein and the recombinational DNA repair of stalled replication forks [J].

Lusetti, SL ;

Cox, MM .

ANNUAL REVIEW OF BIOCHEMISTRY, 2002, 71 :71-100

[23] Characterization of the oligomeric states of RecA protein: Monomeric RecA protein can form a nucleoprotein filament [J].

Masui, R ;

Mikawa, T ;

Kato, R ;

Kuramitsu, S .

BIOCHEMISTRY, 1998, 37 (42) :14788-14797

[24]

MCENTEE K, 1981, J BIOL CHEM, V256, P8835

[25] Molecular design and functional organization of the RecA protein [J].

McGrew, DA ;

Knight, KL .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2003, 38 (05) :385-432

[26] INTERACTION OF RECA PROTEIN WITH SINGLE-STRANDED-DNA - QUANTITATIVE ASPECTS OF BINDING-AFFINITY MODULATION BY NUCLEOTIDE COFACTORS [J].

MENETSKI, JP ;

KOWALCZYKOWSKI, SC .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 181 (02) :281-295

[27] LIGHT-SCATTERING STUDIES OF THE RECA PROTEIN OF ESCHERICHIA-COLI - RELATIONSHIP BETWEEN FREE RECA FILAMENTS AND THE RECA.SSDNA COMPLEX [J].

MORRICAL, SW ;

COX, MM .

BIOCHEMISTRY, 1985, 24 (03) :760-767

[28]

NASTRI HG, 1994, J BIOL CHEM, V269, P26311

[29] Differential rates of NTP hydrolysis by the mutant [S69G]RecA protein - Evidence for a coupling of NTP turnover to DNA strand exchange [J].

Nayak, S ;

Bryant, FR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (37) :25979-25982

[30] CHARACTERISTICS OF PURIFIED RECA-PROTEIN AND THE REGULATION OF ITS SYNTHESIS INVIVO [J].

OGAWA, T ;

WABIKO, H ;

TSURIMOTO, T ;

HORII, T ;

MASUKATA, H ;

OGAWA, H .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1979, 43 :909-915

← 1 2 3 4 5 →