Quantification of hyaluronan in pharmaceutical formulations using high performance capillary electrophoresis and the modified uronic acid carbazole reaction

The amount of hyaluronan (HA) in pharmaceutical formulations was determined by high-performance capillary electrophoresis (HPCE) and the results were compared with the carbazole reaction established by Bitter and Muir (T. Bitter, H.M. Muir, Anal. Biochem. 4 (1962) 330-334), HA analysis was performed in less than 10 min by using an untreated fused silica capillary with bubble detection cell. The influence of several buffers and pH values was examined. Calibration curve shows good linearity from 0.01 to 5.0 mg/ml. The lower limit of detection by monitoring the absorbance at 195 nm was 10 mu g/ml at a signal to noise ratio of 5. (C) 1999 Elsevier Science B.V. All rights reserved.