High resolution studies of hyaluronic acid mixtures through capillary gel electrophoresis

被引：39

作者：

Hong, MF ^{[1
]}

Sudor, J ^{[1
]}

Stefansson, M ^{[1
]}

Novotny, MV ^{[1
]}

机构：

[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 03期

关键词：

D O I：

10.1021/ac970768a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hyaluronic acid is a negatively charged polysaccharide with a high degree of polydispersity that makes the separation of its oligomers extremely difficult. Through the use of columns filled with a highly viscous polyacrylamide matrix, the unit resolution of hyaluronate oligomers could be achieved, up to at least 80 kDa of mass, through capillary electrophoresis. As analytical application examples, the fractions of enzymatically or ultrasonically degraded hyaluronates were monitored through this method. Because of the very high resolving power, peaks additional to the regular oligomers can be observed that are assumed to be conformers of this regular, unbranched biopolymer.

引用

页码：568 / 573

页数：6

共 34 条

[1] THE HYALURONATE RECEPTOR IS PREFERENTIALLY EXPRESSED ON PROLIFERATING EPITHELIAL-CELLS [J].