Class I integrons containing a dhfrI trimethoprim resistance gene cassette in aquatic Acinetobacter spp.

被引：34

作者：

Petersen, A ^{[1
]}

Guardabassi, L ^{[1
]}

Dalsgaard, A ^{[1
]}

Olsen, JE ^{[1
]}

机构：

[1] Royal Vet & Agr Univ, Dept Vet Microbiol, DK-1870 Frederiksberg C, Denmark

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 182卷 / 01期

关键词：

integron; Acinetobacter; gene cassette; antibiotic resistance; aquatic environment;

D O I：

10.1016/S0378-1097(99)00563-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The presence of antibiotic resistance gene cassettes in class I integrons was investigated in 24 sulfamethoxazole-resistant and -sensitive Acinetobacter isolates derived from two Danish freshwater trout farms. Integrons were detected in five isolates from one of the fish farms, and their inserts were characterised by DNA sequencing. Each isolate contained a dhfrI gene cassette encoding resistance to trimethoprim and an open reading frame orfC of unknown function identical to the content of an integron previously found in a clinical enterobacterial isolate. Among the five isolates, at least two different strains were differentiated based on phenotypic tests and randomly amplified polymorphic DNA analysis. To our knowledge, this is the first report and characterisation of an integron in environmental bacteria. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：73 / 76

页数：4

共 31 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] The integrons In0, In2, and In5 are defective transposon derivatives [J].

Brown, HJ ;

Stokes, HW ;

Hall, RM .

JOURNAL OF BACTERIOLOGY, 1996, 178 (15) :4429-4437

[3]

Depaola Angelo, 1995, Journal of Aquatic Animal Health, V7, P155, DOI 10.1577/1548-8667(1995)007&lt

[4]

0155:TRBBIR&gt

[5]

2.3.CO

[6]

[7]

Dijkshoorn L, 1996, ACINETOBACTER MICROB, P37

[8]

Gonzalez G, 1998, FEMS MICROBIOL LETT, V161, P125

[9] AUTOMATED LASER FLUORESCENCE ANALYSIS OF RANDOMLY AMPLIFIED POLYMORPHIC DNA - A RAPID METHOD FOR INVESTIGATING NOSOCOMIAL TRANSMISSION OF ACINETOBACTER-BAUMANNII [J].

GRUNDMANN, H ;

SCHNEIDER, C ;

TICHY, HV ;

SIMON, R ;

KLARE, I ;

HARTUNG, D ;

DASCHNER, FD .

JOURNAL OF MEDICAL MICROBIOLOGY, 1995, 43 (06) :446-451

[10] Multicenter study using standardized protocols and reagents for evaluation of reproducibility of PCR-based fingerprinting of Acinetobacter spp. [J].

Grundmann, HJ ;

Towner, KJ ;

Dijkshoorn, L ;

GernerSmidt, P ;

Maher, M ;

Seifert, H ;

Vaneechoutte, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (12) :3071-3077

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