AUTOMATED LASER FLUORESCENCE ANALYSIS OF RANDOMLY AMPLIFIED POLYMORPHIC DNA - A RAPID METHOD FOR INVESTIGATING NOSOCOMIAL TRANSMISSION OF ACINETOBACTER-BAUMANNII

被引：31

作者：

GRUNDMANN, H

SCHNEIDER, C

TICHY, HV

SIMON, R

KLARE, I

HARTUNG, D

DASCHNER, FD

机构：

[1] TUV ENERGIE & UMWELT GMBH,FACHGRP BIOL SICHERHEIT,D-79108 FREIBURG,GERMANY

[2] ROBERT KOCH INST,D-38855 WERNIGERODE,GERMANY

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 1995年 / 43卷 / 06期

关键词：

D O I：

10.1099/00222615-43-6-446

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid method for genotyping Acinetobacter baumannii based on PCR-fingerprinting with fluorescent primers was evaluated, Automated laser fluorescence analysis (ALFA) enabled on-line generation of high resolution DNA-fingerprints during polyacrylamide gel electrophoresis of randomly amplified polymorphic DNA (RAPD) products. The results were in concordance with macro-restriction fragment patterns produced by pulsed-field gel electrophoresis (PFGE) of ApaI digests of chromosomal DNA. RAPD-ALFA was able to identify homologous strains suggestive of horizontal transmission in < 8 h after colonies were obtained on solid media, whereas PFGE analysis took c. 90 h. Speed and digitised data format renders RAPD-ALFA attractive for routine in-house epidemiological screening of isolates from intensive care and other hospital units.

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页码：446 / 451

页数：6

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