Identification of Streptococcus agalactiae Isolates from Various Phylogenetic Lineages by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

被引：87

作者：

Lartigue, Marie-Frederique ^{[1
,2
]}

Hery-Arnaud, Genevieve ^{[2
]}

Haguenoer, Eve ^{[2
]}

Domelier, Anne-Sophie ^{[1
,2
]}

Schmit, Pierre-Olivier ^{[4
]}

van der Mee-Marquet, Nathalie ^{[1
,2
]}

Lanotte, Philippe ^{[2
]}

Mereghetti, Laurent ^{[2
]}

Kostrzewa, Markus ^{[3
]}

Quentin, Roland ^{[1
,2
]}

机构：

[1] Hop Trousseau, CHRU Tours, Serv Bacteriol & Hyg Hosp, F-37044 Tours, France

[2] Univ Tours, UFR Med, EA Bacteries & Risque Materno Foetal, Inst Federatif Rech Agents Transmissibles & Infec, F-37032 Tours, France

[3] Bruker Daltonik GmbH, D-04318 Leipzig, Germany

[4] Bruker Daltonics Inc, F-67166 Wissembourg, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 07期

关键词：

INVASIVE NEONATAL DISEASE; GROUP-B STREPTOCOCCUS; RAPID IDENTIFICATION; STRAINS; BACTERIA; DESORPTION/IONIZATION; MENINGITIS; SEROTYPE; CLONE;

D O I：

10.1128/JCM.00175-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Variations in proteins related to bacterial diversity may affect species identification performed using matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry. Using this method, we identified 110 Streptococcus agalactiae isolates characterized by serotyping and multilocus sequence typing. Serotype III and sequence type 23 strains expressed the widest variation in molecular weight of putative "species-identifying" biomarker ions. Recognition of the diversity of MALDI patterns observed in strains that represent all major intraspecies lineages assists in the constitution of an optimal reference database.

引用

页码：2284 / 2287

页数：4

共 18 条

[1] Rapid identification and typing of Listeria species by matrix-assisted laser desorption ionization-time of flight mass spectrometry [J].

Barbuddhe, Sukhadeo B. ;

Maier, Thomas ;

Schwarz, Gerold ;

Kostrzewa, Markus ;

Hof, Herbert ;

Domann, Eugen ;

Chakraborty, Trinad ;

Hain, Torsten .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (17) :5402-5407

[2] Genetic diversity of rRNA operons of unrelated Streptococcus agalactiae strains isolated from cerebrospinal fluid of neonates suffering from meningitis [J].

Chatellier, S ;

Huet, H ;

Kenzi, S ;

Rosenau, A ;

Geslin, P ;

Quentin, R .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (11) :2741-2747

[3] Loss of catabolic function in Streptococcus agalactiae strains and its association with neonatal meningitis [J].

Domelier, Anne-Sophie ;

van der Mee-Marquet, Nathalie ;

Grandet, Adeline ;

Mereghetti, Laurent ;

Rosenau, Agnes ;

Quentin, Roland .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (09) :3245-3250

[4] Characterization of intact microorganisms by MALDI mass spectrometry [J].

Fenselau, C ;

Demirev, PA .

MASS SPECTROMETRY REVIEWS, 2001, 20 (04) :157-171

[5] Mobile genetic elements provide evidence for a bovine origin of clonal complex 17 of Streptococcus agalactiae [J].

Hery-Arnaud, Genevieve ;

Bruant, Guillaume ;

Lanotte, Philippe ;

Brun, Stella ;

Picard, Bertrand ;

Rosenau, Agns ;

van der Mee-Marquet, Nathalie ;

Rainard, Pascal ;

Quentin, Roland ;

Mereghetti, Laurent .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (14) :4668-4672

[6]

Holland RD, 1996, RAPID COMMUN MASS SP, V10, P1227, DOI 10.1002/(SICI)1097-0231(19960731)10:10<1227::AID-RCM659>3.0.CO

[7]

2-6

[8] Multilocus sequence typing system for group B streptococcus [J].

Jones, N ;

Bohnsack, JF ;

Takahashi, S ;

Oliver, KA ;

Chan, MS ;

Kunst, F ;

Glaser, P ;

Rusniok, C ;

Crook, DWM ;

Harding, RM ;

Bisharat, N ;

Spratt, BG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (06) :2530-2536

[9] Serotype identification of group B streptococci by PCR and sequencing [J].

Kong, FR ;

Gowan, S ;

Martin, D ;

James, G ;

Gilbert, GL .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (01) :216-226

[10]

Krishnamurthy T, 1996, RAPID COMMUN MASS SP, V10, P883, DOI 10.1002/(SICI)1097-0231(19960610)10:8<883::AID-RCM594>3.3.CO

← 1 2 →