A 4-dimensional representation of antennal lobe output based on an ensemble of characterized projection neurons

被引:13
作者
Staudacher, Erich M. [1 ]
Huetteroth, Wolf [2 ]
Schachtner, Joachim [2 ]
Daly, Kevin C. [1 ]
机构
[1] W Virginia Univ, Dept Biol, Morgantown, WV 26506 USA
[2] Univ Marburg, FB Biol Tierphysiol, D-35043 Marburg, Germany
关键词
Moth; Manduca sexta L; Olfaction; Intracellular recording and staining; Identified glomeruli; 3-Dimensional reconstruction; Ensemble analysis; Spike time resolution; MOTH MANDUCA-SEXTA; FEMALE SPODOPTERA-LITTORALIS; MAMMALIAN OLFACTORY-BULB; SPHINX MOTH; DROSOPHILA-MELANOGASTER; ODOR REPRESENTATIONS; SENSORY MAP; GLOMERULAR REPRESENTATION; HELIOTHIS-VIRESCENS; PLANT VOLATILES;
D O I
10.1016/j.jneumeth.2009.03.019
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A central problem facing studies of neural encoding in sensory systems is how to accurately quantify the extent of spatial and temporal responses. In this study, we take advantage of the relatively simple and stereotypic neural architecture found in invertebrates. We combine standard electrophysiological techniques, recently developed population analysis techniques, and novel anatomical methods to form an innovative 4-dimensional view of odor output representations in the antennal lobe of the moth Manduca sexta. This novel approach allows quantification of olfactory responses of characterized neurons with spike time resolution. Additionally, arbitrary integration windows can be used for comparisons with other methods such as imaging. By assigning statistical significance to changes in neuronal firing, this method can visualize activity across the entire antennal lobe. The resulting 4-dimensional representation of antennal lobe output complements imaging and multi-unit experiments yet provides a more comprehensive and accurate view of glomerular activation patterns in spike time resolution. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:208 / 223
页数:16
相关论文
共 103 条
[21]  
ELJUNDI B, J COMP NEUR IN PRESS
[22]   AN ATLAS AND 3-D RECONSTRUCTION OF THE ANTENNAL LOBES IN THE WORKER HONEY BEE, APIS-MELLIFERA 6 (HYMENOPTERA, APIDAE) [J].
FLANAGAN, D ;
MERCER, AR .
INTERNATIONAL JOURNAL OF INSECT MORPHOLOGY & EMBRYOLOGY, 1989, 18 (2-3) :145-159
[23]   Combinatorial and chemotopic odorant coding in the zebrafish olfactory bulb visualized by optical imaging [J].
Friedrich, RW ;
Korsching, SI .
NEURON, 1997, 18 (05) :737-752
[24]   Dynamic optimization of odor representations by slow temporal patterning of mitral cell activity [J].
Friedrich, RW ;
Laurent, G .
SCIENCE, 2001, 291 (5505) :889-894
[25]   A digital three-dimensional atlas of the honeybee antennal lobe based on optical sections acquired by confocal microscopy [J].
Galizia, CG ;
McIlwrath, SL ;
Menzel, R .
CELL AND TISSUE RESEARCH, 1999, 295 (03) :383-394
[26]   The glomerular code for odor representation is species specific in the honeybee Apis mellifera [J].
Galizia, CG ;
Sachse, S ;
Rappert, A ;
Menzel, R .
NATURE NEUROSCIENCE, 1999, 2 (05) :473-478
[27]   Identification of candidate Drosophila olfactory receptors from genomic DNA sequence [J].
Gao, Q ;
Chess, A .
GENOMICS, 1999, 60 (01) :31-39
[28]   Arm movements in monkeys: Behavior and neurophysiology [J].
Georgopoulos, AP .
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY, 1996, 179 (05) :603-612
[29]  
GEORGOPOULOS AP, 1988, J NEUROSCI, V8, P2928
[30]  
Groh JM, 1997, J NEUROSCI, V17, P4312