Comparison between biochemical and polymerase chain reaction methods for the identification of Lactobacillus spp. isolated from chickens

被引：8

作者：

Barros, M. R. ^{[1
]}

Andreatti Filho, R. L. ^{[1
]}

Oliveira, D. E. ^{[2
]}

Lima, E. T. ^{[1
]}

Crocci, A. J. ^{[3
]}

机构：

[1] UNESP, Fac Med Vet & Zootecnia, BR-18618000 Botucatu, SP, Brazil

[2] UNESP, Fac Med, BR-18618000 Botucatu, SP, Brazil

[3] UNESP, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil

来源：

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2009年 / 61卷 / 02期

关键词：

chicken; broiler breeders; biochemical identification; Lactobacillus; PCR; LACTIC-ACID BACTERIA; RIBOSOMAL-RNA; SP-NOV; ACIDOPHILUS; IDENTIFICATION; TAXONOMY; 16S-23S;

D O I：

10.1590/S0102-09352009000200006

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Lactobacilli were isolated from crops and ceca of broiler breeders and characterized by positive Gram staining, negative catalase test, production of gas from glucose, and negative for H2S production from triple sugar iron, and carbohydrates fermentation. Primers: Lac 1/23-10C for detecting Lactobacillus acidophilus, L. crispatus, L. amylovorus, L. gasseri, L. helveticus, and L. jensenii; Lac 2/LU-1' for L. acidophilus; Fer3/Fer4 for L. fermentum; Reu1/Reu2 for L. reuteri, and Sal1/Sal2 for L. salivarius were used. L. reuteri and L. salivarius were identified by both polymerase chain reaction (PCR) and biochemical tests. However, L. acidophilus, L. fermentum, and Lactobacillus sp. were only identified by biochemical tests. PCR results were more precise, considering the variability of carbohydrate fermentation among the strains, especially for identifying L. fermentum and L. reuteri.

引用

页码：319 / 325

页数：7

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