Characterization and chromosomal location of Pm40 in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium

被引:364
作者
Luo, P. G. [1 ,2 ]
Luo, H. Y. [1 ]
Chang, Z. J. [3 ]
Zhang, H. Y. [1 ]
Zhang, M. [1 ]
Ren, Z. L. [1 ,2 ]
机构
[1] Sichuan Agr Univ, State Key Lab Plant Breeding & Genet, Yaan 625014, Sichuan, Peoples R China
[2] Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Peoples R China
[3] Shanxi Acad Agr Sci, Inst Crop Genet, Taiyuan 030031, Shanxi, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
MOLECULAR CHARACTERIZATION; MARKERS; TRANSLOCATION; LOCI; MAP;
D O I
10.1007/s00122-009-0962-0
中图分类号
S3 [农学(农艺学)];
学科分类号
090104 [作物信息科学与技术];
摘要
Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a very destructive wheat (Triticum aestivum) disease. Resistance was transferred from Elytrigia intermedium to common wheat by crossing and backcrossing, and line GRY19, that was subsequently selected, possessed a single dominant gene for seedling resistance. Five polymorphic microsatellite markers, Xgwm297, Xwmc335, Xwmc364, Xwmc426 and Xwmc476, on chromosome arm 7BS, were mapped relative to the powdery mildew resistance locus in an F-2 population of Mianyang 11/GRY19. The loci order Xwmc426-Xwmc335-Pm40-Xgwm297-Xwmc364-Xwmc476, with 5.9, 0.2, 0.7, 1.2 and 2.9 cM genetic distances, was consistent with published maps. The resistance gene transferred from Elytrigia intermedium into wheat line GRY19 was novel, and was designated Pm40. The close flanking markers will enable marker assisted transfer of this gene into wheat breeding populations.
引用
收藏
页码:1059 / 1064
页数:6
相关论文
共 33 条
[1]
[Anonymous], P 9 INT WHEAT GEN S
[2]
FAST AND SENSITIVE SILVER STAINING OF DNA IN POLYACRYLAMIDE GELS [J].
BASSAM, BJ ;
CAETANOANOLLES, G ;
GRESSHOFF, PM .
ANALYTICAL BIOCHEMISTRY, 1991, 196 (01) :80-83
[3]
Isolation and characterisation of microsatellites from hexaploid bread wheat [J].
Bryan, GJ ;
Collins, AJ ;
Stephenson, P ;
Orry, A ;
Smith, JB ;
Gale, MD .
THEORETICAL AND APPLIED GENETICS, 1997, 94 (05) :557-563
[4]
Molecular characterization of a cryptic wheat-Thinopyrum intermedium translocation line:: evidence for genomic instability in nascent allopolyploid and aneuploid lines [J].
Dong, YS ;
Bu, XL ;
Luan, YS ;
He, MY ;
Liu, B .
GENETICS AND MOLECULAR BIOLOGY, 2004, 27 (02) :237-241
[5]
Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat [J].
Gupta, PK ;
Balyan, HS ;
Edwards, KJ ;
Isaac, P ;
Korzun, V ;
Röder, M ;
Gautier, MF ;
Joudrier, P ;
Schlatter, AR ;
Dubcovsky, J ;
De la Pena, RC ;
Khairallah, M ;
Penner, G ;
Hayden, MJ ;
Sharp, P ;
Keller, B ;
Wang, RCC ;
Hardouin, JP ;
Jack, P ;
Leroy, P .
THEORETICAL AND APPLIED GENETICS, 2002, 105 (2-3) :413-422
[6]
Molecular markers and their applications in wheat breeding [J].
Gupta, PK ;
Varshney, RK ;
Sharma, PC ;
Ramesh, B .
PLANT BREEDING, 1999, 118 (05) :369-390
[7]
Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding [J].
Huang, XQ ;
Hsam, SLK ;
Zeller, FJ ;
Wenzel, G ;
Mohler, V .
THEORETICAL AND APPLIED GENETICS, 2000, 101 (03) :407-414
[8]
Järve K, 2000, GENOME, V43, P377, DOI 10.1139/gen-43-2-377
[9]
Kosambi D. D., 1944, ANN EUGENICS, V12, P172
[10]
A cryptic wheat-Aegilops triuncialis translocation with leaf rust resistance gene Lr58 [J].
Kuraparthy, Vasu ;
Sood, Shilpa ;
Chhuneja, Parveen ;
Dhaliwal, Harcharan S. ;
Kaur, Satinder ;
Bowden, Robert L. ;
Gill, Bikram S. .
CROP SCIENCE, 2007, 47 (05) :1995-2003