Application of quantum dot - Antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue

被引：58

作者：

Ding, Shuangyang

Chen, Junxia

Jiang, Haiyang

He, Jihong

Shi, Weimin

Zhao, Wenshen

Shen, Jianzhong ^{[1
]}

机构：

[1] China Agr Univ, Coll Vet Med, Dept Vet Pharmacol & Toxicol, Beijing 100094, Peoples R China

[2] China Agr Univ, Coll Agr & Biotechnol, Dept Plant Pathol, Beijing 100094, Peoples R China

[3] China Anim Husbandry Ind Co Ltd, Beijing 100070, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 17期

关键词：

quantum dots; fluorescence immunoassay; sulfamethazine; chicken muscle;

D O I：

10.1021/jf0606961

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A new indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method for the detection of sulfamethazine (SM2) in chicken muscle tissue was demonstrated using quantum dots (QDs) as the fluorescence label coupled with secondary antibody. The sensitivity of the cFLISA was compared with that of the HPLC method. The 50% inhibition value (IC50) and the limit of detection (LOD) of the cFLISA were 7.7 and 1.0 ng/mL, respectively. When SM2 was spiked at levels of 50, 100, and 200 ng/ g, recoveries ranged from 80.6% to 117.4%, with a coefficient of variation of 6.9-9.6%. In the incurred sample analysis, the amounts of SM2 quantified by cFLISA were similar to the results obtained by the HPLC method. This study shows that cFLISA could be used as a screening method in practice.

引用

页码：6139 / 6142

页数：4

共 17 条

[11] Multiplexed toxin analysis using four colors of quantum dot fluororeagents [J].