Mild hypothermia protects obese rats from fulminant hepatic necrosis induced by ischemia-reperfusion

被引:27
作者
Niemann, Claus U.
Choi, Soojinna
Behrends, Matthias
Hirose, Ryutaro
Noh, Joonhwa
Coatney, John L.
Roberts, John R.
Serkova, Natalie J.
Maher, Jacquelyn J.
机构
[1] Univ Calif San Francisco, Dept Anesthesia & Perioperat, Div Liver Transplantat, San Francisco, CA 94143 USA
[2] Chonnam Natl Univ, Sch Med, Kwangju, South Korea
[3] Kwangju Christian Hosp, Dept Urol, Kwangju, South Korea
[4] Univ Calif San Francisco, Dept Surg, Div Transplantat, San Francisco, CA 94143 USA
[5] Univ Klinikum Essen, Klin Anasthesiol & Instensivmed, Essen, Germany
[6] Univ Colorado, Hlth Sci Ctr, Dept Anesthesiol, Biomed MRI MRS, Denver, CO USA
[7] Univ Calif San Francisco, Rice Liver Ctr Lab, San Francisco, CA 94143 USA
[8] Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
关键词
D O I
10.1016/j.surg.2006.01.021
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background. Obese Zucker rats demonstrate increased susceptibility to hepatic ischemia-reperfusion injury. This study evaluates the effect of mild systemic hypothermia on ischemia-induced acute fulminant necrosis during warm ischemia and reperfusion, and investigates blood metabolic profiles, under normothermic and mildly hypothermic conditions. Methods. The-left and median hepatic lobes of male, obese, Zucker rats were exposed to 75 minutes of ischemia under either normothermic (36.9 +/- 0.3 degrees C) or mildly hypothermic (33.3 +/- 0.1 degrees C) conditions followed by 8 hours of reperfusion. Animals were killed and tissue and blood were harvested for analysis of histology, liver enzymes, and metabolic H-1-NMR spectroscopy. Results. Liver enzyme activities were significantly higher in the normothermic group when compared with. mildly hypothermic animals. Histologic analysis showed greater than 75% necrosis in the normothemic group, whereas in the mildly hypothermic g-roup necrosis was less than 25%. Blood from of lactate (190%, P = .001) and lower normothermic animals contained greater concentrations of concentrations of glucose (60%, P = .01) than hypothermic animals; hepatic osmolyte betaine was also increased in blood from the normothermic group (220 %, P = .0002). In addition, normothermic rats had increased concentrations of circulating fatty acids, triglycerides, glutamate, succinate, and acetate when compared with the hypothermic. Conclusion. Mild hypothermia decreased hepatic necrosis in obese rats. NMR blood profiles indicate that hypothermia protects hepatic metabolism.
引用
收藏
页码:404 / 412
页数:9
相关论文
共 43 条
  • [41] Historical evolution of hypothermic liver surgery
    Yamanaka, N
    Dal, CL
    Okamoto, E
    [J]. WORLD JOURNAL OF SURGERY, 1998, 22 (10) : 1104 - 1107
  • [42] Evolution of intrahepatic carbon, nitrogen, and energy metabolism in a D-galactosamine-induced rat liver failure model
    Yokoyama, T
    Banta, S
    Berthiaume, F
    Nagrath, D
    Tompkins, RG
    Yarmush, ML
    [J]. METABOLIC ENGINEERING, 2005, 7 (02) : 88 - 103
  • [43] Mild hypothermia protects against postischemic hepatic endothelial injury and decreases the formation of reactive oxygen species
    Zar, HA
    Lancaster, JR
    [J]. REDOX REPORT, 2000, 5 (05) : 303 - 310