The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly

被引:125
作者
Follit, John A.
Tuft, Richard A.
Fogarty, Kevin E.
Pazour, Gregory J. [1 ]
机构
[1] Univ Massachusetts, Sch Med, Dept Physiol, Program Mol Med, Worcester, MA 01605 USA
[2] Univ Massachusetts, Sch Med, Dept Physiol, Biomed Imaging Grp, Worcester, MA 01605 USA
关键词
D O I
10.1091/mbc.e06-02-0133
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT particles are composed of at least 17 polypeptides that are thought to contain binding sites for various cargos that need to be transported from their site of synthesis in the cell body to the site of assembly in the cilium. We show here that the IFT20 subunit of the particle is localized to the Golgi complex in addition to the basal body and cilia where all previous IFT particle proteins had been found. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. Strong knock down of IFT20 in mammalian cells blocks ciliary assembly but does not affect Golgi structure. Moderate knockdown does not block cilia assembly but reduces the amount of polycystin-2 that is localized to the cilia. This work suggests that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium.
引用
收藏
页码:3781 / 3792
页数:12
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