Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae

被引：519

作者：

Wieczorke, R ^{[1
]}

Krampe, S ^{[1
]}

Weierstall, T ^{[1
]}

Freidel, K ^{[1
]}

Hollenberg, CP ^{[1
]}

Boles, E ^{[1
]}

机构：

[1] Univ Dusseldorf, Inst Mikrobiol, D-40225 Dusseldorf, Germany

来源：

FEBS LETTERS | 1999年 / 464卷 / 03期

关键词：

functional analysis; gene family; sugar transport; multiple deletion; yeast; gene redundancy;

D O I：

10.1016/S0014-5793(99)01698-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hexose transporter family of Saccharomyces cerevisiae comprises 18 proteins (Hxt1-17, Gal2), Here, we demonstrate that all these proteins, except Hxt12, and additionally three members of the maltose transporter family (Agt1, Yd1247, Yjr160) are able to transport hexoses, In a yeast strain deleted for HXT1-17, GAL2, AGT1, YDL247w and YJR160c, glucose consumption and transport activity were completely abolished. However, as additional deletion of the glucose sensor gene SNF3 partially restored growth on hexoses, our data indicate the existence of even more proteins able to transport hexoses in yeast. (C) 1999 Federation of European Biochemical Societies.

引用

页码：123 / 128

页数：6

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