Selection of DNA-Encoded Small Molecule Libraries Against Unmodified and Non-Immobilized Protein Targets

被引：87

作者：

Zhao, Peng ^{[5
]}

Chen, Zitian ^{[6
]}

Li, Yizhou ^{[5
]}

Sun, Dawei ^{[5
]}

Gao, Yuan ^{[5
]}

Huang, Yanyi ^{[3
,4
]}

Li, Xiaoyu ^{[1
,2
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China

[2] Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Key Lab Chem Genom, Shenzhen 518055, Peoples R China

[3] Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, Beijing 100871, Peoples R China

[4] Peking Univ, Coll Engn, Beijing 100871, Peoples R China

[5] Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China

[6] Peking Univ, Coll Engn, Beijing 100871, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 38期

关键词：

DNA; DNA-encoded library; DNA-templated chemistry; drug discovery; selection; IN-VITRO SELECTION; TERMINAL PROTECTION; BINDING PROTEINS; PEPTIDE LIBRARY; LINKED DNA; DISCOVERY; IDENTIFICATION; ASSAY; PCR; EXONUCLEASE;

D O I：

10.1002/anie.201404830

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The selection of DNA-encoded libraries against biological targets has become an important discovery method in chemical biology and drug discovery, but the requirement of modified and immobilized targets remains a significant disadvantage. With a terminal protection strategy and ligand-induced photo-crosslinking, we show that iterated selections of DNA-encoded libraries can be realized with unmodified and non-immobilized protein targets.

引用

页码：10056 / 10059

页数：4

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