Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli

被引：129

作者：

Yamamoto, Kaneyoshi ^{[1
]}

Ishihama, Akira

机构：

[1] Kinki Univ, Dept Adv Biosci, Nakama, Nara 6318505, Japan

[2] Nippon Inst Biol Sci, Div Mol Biol, Tokyo 1980024, Japan

[3] Hosei Univ, Fac Engn, Koganei, Tokyo 1848584, Japan

[4] Hosei Univ, Res Ctr Micro Nanotechnol, Koganei, Tokyo 1848584, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 07期

关键词：

transcription regulation; CpxA/CpxR; two-component system; copper response; Escherichia coli; 2-COMPONENT SIGNAL-TRANSDUCTION; ENVELOPE STRESS-RESPONSE; TRANSCRIPTIONAL RESPONSE; CPXR-P; PATHWAY; SYSTEM; GENE; INDUCTION; DEGP; OVERPRODUCTION;

D O I：

10.1271/bbb.60024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The copper stimulon in Escherichia coli consists of four regulons, the CueR-, CusS/CusR-, CpxA/CpxR-, and YedV/YedW regulons. E. coli mutants defective in cpxRA showed higher sensitivity to copper than the wild type. A total of 15 promoters were found to be induced in E. coli culture upon exposure to copper in a CpxA/ CpxR-dependent manner. After gel-shift and DNase I foot-printing analyses, a conserved tandem repeat of pentanucleotide sequence, GTAAA(N)(4-8)GTAAA, with a conserved A of 4-bp upstream of each pentamer, was identified to be the CpxR-binding site. The difference in the orientation and location of the CpxR box is discussed with respect to the regulation mechanism among CpxR-regulon genes.

引用

页码：1688 / 1695

页数：8

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