New chemistry of BODIPY dyes, and BODIPY dye cassettes featuring through-bond energy transfer

被引：5

作者：

Burgess, K ^{[1
]}

Burghart, A ^{[1
]}

Chen, J ^{[1
]}

Wan, CW ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, College Stn, TX 77842 USA

来源：

ADVANCES IN NUCLEIC ACID AND PROTEIN ANALYSES, MANIPULATION, AND SEQUENCING | 2000年 / 1卷

关键词：

DNA sequencing; energy transfer; BODIPY; cassettes; fluorescence;

D O I：

10.1117/12.380500

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We are attempting to design and prepare a new class of dyes for biomedical/biotechnological applications, including detection of oligonucleotides in DNA sequencing and diagnostics. The dyes use through-bond energy transfer to harvest short wavelength irradiation, and fluoresce at much longer wavelengths. Excellent resolution of fluorescent emissions from a 266 nm laser source is observed, with apparent Stokes' shifts of up to 408 nm.

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收藏

页码：95 / 105

页数：11

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