Diffraction phase and fluorescence microscopy

被引：211

作者：

Park, YongKeun ^{[1
]}

Popescu, Gabriel

Badizadegan, Kamran

Dasari, Ramachandra R.

Feld, Michael S.

机构：

[1] MIT, George R Harrison Spect Lab, Cambridge, MA 02139 USA

[2] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02114 USA

[3] Massachusetts Gen Hosp, Boston, MA 02114 USA

来源：

OPTICS EXPRESS | 2006年 / 14卷 / 18期

关键词：

D O I：

10.1364/OE.14.008263

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have developed diffraction phase and fluorescence (DPF) microscopy as a new technique for simultaneous quantitative phase imaging and epi-fluorescence investigation of live cells. The DPF instrument consists of an interference microscope, which is incorporated into a conventional inverted fluorescence microscope. The quantitative phase images are characterized by sub-nanometer optical path-length stability over periods from milliseconds to a cell lifetime. The potential of the technique for quantifying rapid nanoscale motions in live cells is demonstrated by experiments on red blood cells, while the composite phase-fluorescence imaging mode is exemplified with mitotic kidney cells. (c) 2006 Optical Society of America

引用

页码：8263 / 8268

页数：6

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