Differential constitutive and activation-dependent expression of prion protein in human peripheral blood leucocytes

被引:73
作者
Dürig, J
Giese, A
Schulz-Schaeffer, W
Rosenthal, C
Schmücker, U
Bieschke, J
Dührsen, U
Kretzschmar, HA
机构
[1] Univ Gottingen, Inst Neuropathol, D-37075 Gottingen, Germany
[2] Univ Hosp Essen, Dept Haematol, D-45122 Essen, Germany
[3] Max Planck Inst Biophys Chem, Dept Biochem Kinet, D-37077 Gottingen, Germany
关键词
flow cytometry; T cells; monocytes; IFN-gamma; PrPC expression;
D O I
10.1046/j.1365-2141.2000.01881.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The cellular isoform of the prion protein (PrPC) is a cell surface glycoprotein that has recently been shown to play a role in haemopoietic cell activation and proliferation. We have characterized the constitutive expression of PrPC on human peripheral blood (pB) cell populations, using PrP-specific antibodies in a multiparameter flow cytometry approach. We found that T cells, NK cells and monocytes exhibit similar PrPC levels, whereas PrPC surface staining on B cells was significantly lower and was virtually absent on granulocytes. Within the T-cell compartment, CD8+ cells showed a significantly higher PrPC expression than CD4+ cells. Similarly, CD3+ cells co-expressing the activation marker CD56 (N-CAM) exhibited significantly higher PrPC expression levels than their CD56- counterparts. Culture of CD14+ pB monocytes for 12-48 h in the presence of interferon gamma (IFN-gamma) resulted in a significant increase in PrPC expression in a time- and concentration-dependent manner. This effect was partially abrogated by the addition of the metabolic inhibitor cycloheximide, indicating the role of protein synthesis in this process. Our results show that PrPC expression on human haemopoietic cells correlates with the activation and developmental status of these cells, suggesting an important functional role of PrPC in the haemopoietic system.
引用
收藏
页码:488 / 496
页数:9
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