Transposon tagging in rice

被引：84

作者：

Izawa, T

Ohnishi, T

Nakano, T

Ishida, N

Enoki, H

Hashimoto, H

Itoh, K

Terada, R

Wu, CY

Miyazaki, C

Endo, T

Iida, S

Shimamoto, K

机构：

[1] NARA INST SCI & TECHNOL, PLANT MOL GENET LAB, NARA 63001, JAPAN

[2] PLANTECH RES INST, AOBA KU, YOKOHAMA, KANAGAWA 227, JAPAN

[3] SCI UNIV TOKYO, DEPT BIOL SCI & TECHNOL, NODA, CHIBA 278, JAPAN

[4] NATL INST BASIC BIOL, OKAZAKI, AICHI 444, JAPAN

[5] NIIGATA UNIV, DEPT AGR, NIIGATA 95021, JAPAN

[6] NATL INST AGROBIOL RESOURCES, TSUKUBA, IBARAKI 305, JAPAN

[7] JAPAN SCI & TECHNOL COOPERAT, NAGASAKI LAB, OHMURA 856, JAPAN

[8] NATL INST FRUIT TREE SCI, SHIMIZU, SHIZUOKA 42402, JAPAN

来源：

PLANT MOLECULAR BIOLOGY | 1997年 / 35卷 / 1-2期

关键词：

rice; Ac/Ds; transposon; gene isolation; disease resistance; flowering time;

D O I：

10.1023/A:1005769605026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To develop an efficient gene isolation method for rice we introduced the maize Ac/Ds system into rice. Extensive analysis of their behavior in rice for several generations indicated that Ac and Ds in the presence of Ac transposase gene actively transpose in rice. A wide spectrum of mutations affecting growth, morphogenesis, flowering time and disease resistance have been obtained in the population carrying Ac/Ds and some of them were genetically analyzed. Main efforts are currently being made to isolate genes responsible these mutations. In addition, a number of Ac/Ds were mapped on chromosomes and mapped elements will be used in the future for directed tagging of genes with known chromosomal positions.

引用

页码：219 / 229

页数：11

共 42 条

[1] TRANSPOSON TAGGING OF A MALE-STERILITY GENE IN ARABIDOPSIS [J].