Transcriptome analysis of the salivary glands of the female tick Amblyomma americanum (Acari: Ixodidae)

被引：48

作者：

Aljamali, M. N. ^{[1
]}

Hern, L. ^{[3
]}

Kupfer, D. ^{[3
]}

Downard, S. ^{[3
]}

So, S. ^{[3
]}

Roe, B. A. ^{[3
]}

Sauer, J. R. ^{[2
]}

Essenberg, R. C. ^{[1
]}

机构：

[1] Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA

[2] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA

[3] Univ Oklahoma, Dept Chem, Norman, OK 73019 USA

来源：

INSECT MOLECULAR BIOLOGY | 2009年 / 18卷 / 02期

关键词：

female tick; salivary glands; Ambyomma americanum; expressed sequence tags; LONE STAR TICK; PLATELET-AGGREGATION INHIBITORS; EXPRESSED SEQUENCE TAGS; IXODES-SCAPULARIS; CATTLE TICK; MOLECULAR-CLONING; PROTEIN; GENE; SIALOME; EXTRACT;

D O I：

10.1111/j.1365-2583.2009.00863.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Ticks infest a wide range of hosts while bypassing their immune, inflammatory and haemostatic responses during their extended feeding, which may last for more than two weeks. Here, we present a transcriptome analysis of 3868 expressed sequence tags (ESTs) from three cDNA libraries generated from the salivary glands of adult female Ambyomma americanum ticks at different stages of feeding. We applied a normalization step for one library, significantly decreasing the abundance of mitochondrial sequences amongst the 2292 sequences from the normalized library. Our ESTs include homologues that may modulate haemostatic, immune and inflammatory responses of the hosts. Other ESTs probably represent important components of the highly efficient secretory pathways for salivary proteins and concomitantly transmitted pathogens.

引用

页码：129 / 154

页数：26

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