Octadecanoid signaling component "burst" in rice (Oryza sativa L.) seedling leaves upon wounding by cut and treatment with fungal elicitor chitosan

被引:82
作者
Rakwal, R
Tamogami, S
Agrawal, GK
Iwahashi, H
机构
[1] AIST, Res Inst Biol Resources, Mol & Microbial Ecol Res Grp, Tsukuba, Ibaraki 3058566, Japan
[2] Akita Prefectural Univ, Dept Reprod Biol, Lab Growth Regulat Chem, Akita 0100195, Japan
[3] RLABB, Kathmandu, Nepal
基金
日本学术振兴会;
关键词
defense/stress response; JA biosynthesis; LC/MS/MS; OPDA; quantification; rice;
D O I
10.1016/S0006-291X(02)00779-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Octadecanoid pathway components, 12-oxo-phytodieonic acid (OPDA) and jasmonic acid (JA), are key biologically active regulators of plant self-defense response(s). However, to date these compounds have been studied mostly in dicots, and used large (1-10 g fresh weight, FW) samples for quantification, even when examined in mature rice plants, which is a drawback considering their rapid responsiveness to stress. Focusing on rice-a monocot cereal crop research model-this work describes an efficient and simultaneous quantification of both OPDA and JA using a minimum amount of 200 mg FW seedling leaf tissue upon wounding (by cut) and treatment with fungal elicitor, chitosan (CT) by high-pressure liquid chromatography-turboionspray tandem mass spectrometry. Transient OPDA/JA "burst" was consistently and reproducibly detected within 3 min in wounded and CT treated leaves. OPDA peaked dramatically around 5 min and returned to its basal level within 15 min, whereas JA induction upon wounding and CT treatment were in parallel to OPDA production, peaking at 30 and 60 min, respectively. Present results mark a major advance in our understanding of key inducible octadecanoid pathway components in rice, and strongly suggest a role for the octadecanoid pathway downstream of perception of at least these two fundamentally different extracellular stimuli. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:1041 / 1045
页数:5
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