Molecular characterization of a mouse heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its tissue-specific expression

被引:21
作者
Akagi, T
Kamei, D
Tsuchiya, N
Nishina, Y
Horiguchi, H
Matsui, M
Kamma, H
Yamada, M
机构
[1] Yokohama City Univ, Grad Sch Integrated Sci, Yokohama, Kanagawa 2360027, Japan
[2] Ibaraki Prefectural Univ Hlth Sci, Ibaraki, Osaka 3000331, Japan
[3] Univ Tsukuba, Inst Basic Med Sci, Tsukuba, Ibaraki 3058575, Japan
关键词
brain; cDNA sequence; HL-60; cells; RNA binding protein; testis;
D O I
10.1016/S0378-1119(00)00032-9
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The human DNA- and RNA-binding protein JKTBP is a new member of heterogeneous nuclear ribonucleoproteins (hnRNPs) that are involved in mRNA biogenesis. We cloned and characterized a mouse homolog and studied its expression in mouse tissues. The cDNA encoded a 301-residue polypeptide. There is only a single amino acid difference between the mouse and human sequences. Northern blotting indicated ubiquitous but varied expressions of approximately 1.4 and 2.8 kb mRNAs in various tissues. Immunoblotting indicated that the amounts of protein of about 38 kDa were higher in the brain and testis than in other tissues. An additional protein or about 53 KDa was found in the brain and testis. Germ cell-deficient W/W-v mutant mice and aged mice had the reduced amounts of JKTBP in the testes. Immunohistochemical staining indicated cell type-specific expression of JKTBP in tissues: neurons and spermatocytes displayed strong signal intensities. The signals were confined to the nucleus. The amount of 38 kDa JKTBP was estimated to be approximately 1.3 x 10(7) molecules per HL-60 cell. These results indicate that JKTBP is an abundant, highly conserved nuclear protein. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:267 / 273
页数:7
相关论文
共 31 条
[31]   The roles of heterogeneous nuclear ribonucleoproteins (hnRNP) in RNA metabolism [J].
Weighardt, F ;
Biamonti, G ;
Riva, S .
BIOESSAYS, 1996, 18 (09) :747-756