Hormone-regulated inflorescence induction and TFL1 expression in Arabidopsis callus in vitro

被引：23

作者：

Guan, C. M. ^{[1
]}

Zhu, S. S. ^{[1
]}

Li, X. G. ^{[1
]}

Zhang, X. S. ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Life Sci, Shandong Key Lab Crop Biol, Shandong 271018, Peoples R China

来源：

PLANT CELL REPORTS | 2006年 / 25卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Arabidopsis thaliana; callus; hormones; inflorescence induction;

D O I：

10.1007/s00299-006-0165-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

To study hormone-regulated inflorescence development, we established the in vitro regeneration system of Arabidopsis inflorescences in the presence of cytokinin and auxin. Media containing a combination of thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) were used to induce callus formation. Higher frequencies of calli were obtained by using the inflorescence stems as explants. After transferring the calli to media containing a combination of zeatin and indole-3-acetic acid (IAA), the inflorescences were induced from the calli. The morphology of regenerated inflorescences was similar to that of inflorescences in plants; however, flowers of regenerated inflorescences often lacked a few floral organs. Furthermore, TFL1, a gene involved in floral transition in Arabidopsis, was activated during the inflorescence induction. Our results suggest that the TFL1 gene plays an important role in hormone-regulated inflorescence formation.

引用

页码：1133 / 1137

页数：5

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