Post-derivatization procedure for determination of hippuric acid after extraction by an automated micro solid phase extraction system and monitoring by gas chromatography

被引：44

作者：

Ahmadi, F. ^{[1
]}

Asgharloo, H. ^{[1
]}

Sadeghi, S. ^{[1
]}

Gharehbagh-Aghababa, V. ^{[1
]}

Adibi, H. ^{[1
]}

机构：

[1] Kermanshah Univ Med Sci, Dept Med Chem, Fac Pharm, Kermanshah 671451673, Iran

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 27期

关键词：

Gas chromatography; Derivatization; Hippuric acid; Micro solid phase extraction; Urine sample; PARA-METHYLHIPPURIC ACIDS; SPECTROMETRY ANALYSIS; PHENYLGLYOXYLIC ACID; TRANS-CINNAMALDEHYDE; FLAME IONIZATION; ESSENTIAL OILS; CINNAMIC ACID; MANDELIC-ACID; HUMAN URINE; MICROEXTRACTION;

D O I：

10.1016/j.jchromb.2009.06.036

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

A rapid, simple and high sensitive method is described here for extraction of hippuric acid (HA) from human urine samples by using an automated micro solid phase extraction system (mu-SPE). However in order to increase sensitivity of gas chromatography with flame ionization detector, a post derivatization procedure was developed. In this work, a polypyrrole was synthesized by chemical oxidation of the pyrrole monomer in non-aqueous solution and applied as an excellent and efficient sorbent for mu-SPE. The calibration curves were linear in the range of 0.018-8.95 mu g mL(-1) for HA, in both water and urine samples with correlation coefficients 0.9973 and 0.9946, respectively; limits of detections were 12.1 ng mL(-1) and 16.5 ng mL(-1), respectively. This method was successfully used to analyze trace amounts of HA in human urine samples without any interference from coexisting substances. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：2945 / 2951

页数：7

共 59 条

[1]

Automated extraction and preconcentration of multiresidue of pesticides on a micro-solid-phase extraction system based on polypyrrole as sorbent and off-line monitoring by gas chromatography-flame ionization detection [J].