Identification of a human endonuclease complex reveals a link between tRNA splicing and Pre-mRNA 3′ end formation

被引：196

作者：

Paushkin, SV ^{[1
]}

Patel, M ^{[1
]}

Furia, BS ^{[1
]}

Peltz, SW ^{[1
]}

Trotta, CR ^{[1
]}

机构：

[1] PTC Therapeut, S Plainfield, NJ 07080 USA

来源：

CELL | 2004年 / 117卷 / 03期

关键词：

D O I：

10.1016/S0092-8674(04)00342-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

tRNA splicing is a fundamental process required for cell growth and division. The first step in tRNA splicing is the removal of introns catalyzed in yeast by the tRNA splicing endonuclease. The enzyme responsible for intron removal in mammalian cells is unknown. We present the identification and characterization of the human tRNA splicing endonuclease. This enzyme consists of HsSen2, HsSen34, HsSen15, and HsSen54, homologs of the yeast tRNA endonuclease subunits. Additionally, we identified an alternatively spliced isoform of SEN2 that is part of a complex with unique RNA endonuclease activity. Surprisingly, both human endonuclease complexes are associated with pre-mRNA3' end processing factors. Furthermore, siRNA-mediated depletion of SEN2 exhibited defects in maturation of both pre-tRNA and pre-mRNA. These findings demonstrate a link between pre-tRNA splicing and pre-mRNA 3' end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.

引用

页码：311 / 321

页数：11

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