Cysteine 116 participates in intermolecular bonding of the human VEGF121 homodimer

被引：7

作者：

Gaspar, NJ ^{[1
]}

Jue, RA ^{[1
]}

Hu, J ^{[1
]}

Puchacz, E ^{[1
]}

deForest, NL ^{[1
]}

Schellenberger, U ^{[1
]}

机构：

[1] Scios Inc, Sunnyvale, CA 94085 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2002年 / 404卷 / 01期

关键词：

VEGF; disulfide characterization; mass spectrometry;

D O I：

10.1016/S0003-9861(02)00239-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

VEGF(121), the 121-amino acid form of vascular endothelial growth factor is a homodimer with nine cysteine residues per monomer. While three intramolecular and two intermolecular disulfide bonds have been mapped, the state of the ninth cysteine, Cys116, is not known. In this study, we determined that human VEGF(121) contains a third interchain disulfide bond between Cys116 of each monomer. We also isolated a VEGF(121) variant with two extra cysteines bound to each Cys116. No evidence was found for the exsistence of Cys116 in the reduced state. In fact, selective reduction of the Cys116 interchain disulfide bond yielded an unstable VEGF(121) molecule, which reoxidized quickly. Biological activities of VEGF(121) Cys116 variants were assessed. The oxidative state of Cys116 has no effect on binding or proliferation activities but may be important for overall stability of the molecule. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：126 / 135

页数：10

共 21 条

[1]

Christinger HW, 1996, PROTEINS, V26, P353, DOI 10.1002/(SICI)1097-0134(199611)26:3<353::AID-PROT9>3.0.CO

[2]

2-E

[3]

HIGH-LEVEL EXPRESSION OF TETANUS TOXIN FRAGMENT-C IN PICHIA-PASTORIS STRAINS CONTAINING MULTIPLE TANDEM INTEGRATIONS OF THE GENE [J].