Purification and characterization of β-agarase from marine bacterium Bacillus cereus ASK202

被引:32
作者
Kim, BJ
Kim, HJ
Ha, SD
Hwang, SH
Byun, DS
Lee, TH
Kong, JK
机构
[1] Pukyong Natl Univ, Fac Food Sci & Biotechnol, Pusan 608737, South Korea
[2] Pusan Natl Univ, Coll Nat Sci, Dept Microbiol, Pusan 609735, South Korea
关键词
beta-agarase; Bacillus cereus ASK202; neoagaro-oligosaccharide;
D O I
10.1023/A:1005604618242
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Extracellular agarase of Bacillus cereus ASK202 was purified 32-fold, giving a single band on PAGE with activity staining. The M-r of purified agarase was determined as 90 kDa by SDS-PAGE. The N-terminal amino acid was sequenced and the sequence did not show homology to any other known agarases. The optimum pH and temperature were 7.0 and 40 degrees C, respectively. This enzyme was found to be a beta-agarase which catalyzed the hydrolysis of the beta-1,4 linkage of agarose to yield neoagarohexaose, neoagarotetraose and neoagarobiose.
引用
收藏
页码:1011 / 1015
页数:5
相关论文
共 16 条
[1]   PURIFICATION AND CHARACTERIZATION OF A NOVEL BETA-AGARASE FROM VIBRIO SP AP-2 [J].
AOKI, T ;
ARAKI, T ;
KITAMIKADO, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 187 (02) :461-465
[2]   CLONING AND GENE REPLACEMENT MUTAGENESIS OF A PSEUDOMONAS-ATLANTICA AGARASE GENE [J].
BELAS, R ;
BARTLETT, D ;
SILVERMAN, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (01) :30-37
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   THE AGARASE GENE (DAGA) OF STREPTOMYCES-COELICOLOR A3(2) - NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL ANALYSIS [J].
BUTTNER, MJ ;
FEARNLEY, IM ;
BIBB, MJ .
MOLECULAR & GENERAL GENETICS, 1987, 209 (01) :101-109
[5]   THIN-LAYER CHROMATOGRAPHIC ANALYSIS OF ENZYMIC HYDROLYSATES OF AGAR [J].
DUCKWORTH, M ;
YAPHE, W .
JOURNAL OF CHROMATOGRAPHY, 1970, 49 (03) :482-+
[6]   Cloning and expression of an agarase gene from a marine bacterium Pseudomonas sp w7 [J].
Kong, JY ;
Hwang, SH ;
Kim, BJ ;
Bae, SK ;
Kim, JD .
BIOTECHNOLOGY LETTERS, 1997, 19 (01) :23-26
[7]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[8]  
LEE HW, 1997, KOREAN J BIOTECHNOL, V12, P228
[9]   PURIFICATION AND PROPERTIES OF AN EXTRACELLULAR AGARASE FROM ALTEROMONAS SP STRAIN-C-1 [J].
LEON, O ;
QUINTANA, L ;
PERUZZO, G ;
SLEBE, JC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (12) :4060-4063
[10]   BETA-AGARASE-I AND BETA-AGARASE-II FROM PSEUDOMONAS-ATLANTICA - PURIFICATIONS AND SOME PROPERTIES [J].
MORRICE, LM ;
MCLEAN, MW ;
WILLIAMSON, FB ;
LONG, WF .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 135 (03) :553-558