Stabilization of invertase by modification of sugar chains with chitosan

被引：64

作者：

Gómez, L ^{[1
]}

Ramírez, HL ^{[1
]}

Villalonga, R ^{[1
]}

机构：

[1] Univ Matanzas, Fac Agron, Ctr Biotechnol Studies, Matanzas, Cuba

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 05期

关键词：

chitosan; enzyme stability; invertase; modified enzyme;

D O I：

10.1023/A:1005664432575

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chitosan was linked to invertase by covalent conjugation to periodate-activated carbohydrate moieties of the enzyme. The thermostability of modified enzyme was enhanced by about 10 degrees C. The half-life at 65 degrees C was increased from 5 min to 5 h. The enzyme stability was enhanced by 20% at pH below 3.0. The half-life of denaturation by 6 M urea was increased by 2 h.

引用

页码：347 / 350

页数：4

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