学术探索
学术期刊
新闻热点
数据分析
智能评审

IN VITRO STUDIES OF LANTIBIOTIC BIOSYNTHESIS

被引：23
作者：
Li, Bo [1 ,2 ,3 ]
Cooper, Lisa E. [1 ,2 ,3 ]
van der Donk, Wilfred A. [1 ,2 ,3 ]
机构：
[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA
[3] Univ Illinois, Howard Hughes Med Inst, Urbana, IL 61801 USA
来源：
COMPLEX ENZYMES IN MICROBIAL NATURAL PRODUCT BIOSYNTHESIS, PART A: OVERVIEW ARTICLES AND PEPTIDES | 2009年 / 458卷
关键词：
SITE-DIRECTED MUTAGENESIS; AMINO-ACID SUBSTITUTIONS; PRECURSOR LIPID II; LACTICIN-481; SYNTHETASE; LEADER PEPTIDE; POSTTRANSLATIONAL MODIFICATION; PEPTIDOGLYCAN BIOSYNTHESIS; OXIDATIVE DECARBOXYLATION; SUBSTRATE-SPECIFICITY; NISIN BIOSYNTHESIS;
D O I：
10.1016/S0076-6879(09)04821-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The lantibiotics are ribosomally synthesized and posttranslationally modified peptide antibiotics containing the thioether crosslinks lanthionine (Lan) and 3-methyllanthionine (MeLan) and typically also the dehydroamino acids dehydroalanine (Dha) and (Z)-dehydrobutyrine (Dhb). These modifications are formed by dehydration of Ser/Thr residues to produce the Dha and Dhb structures, and subsequent conjugate additions of Cys residues onto the unsaturated amino acids to form thioether rings (Lan and MeLan). Several of the enzymatic reactions involved in lantibiotic biosynthesis have been reconstituted in vitro in recent years and these systems as well as a general overview of lantibiotic biosynthesis are discussed in this chapter.
引用
收藏
页码：533 / +
页数：27
相关论文
共 74 条
[1]   Crystal structure of the peptidyl-cysteine decarboxylase EpiD complexed with a pentapeptide substrate [J].
Blaesse, M ;
Kupke, T ;
Huber, R ;
Steinbacher, S .
EMBO JOURNAL, 2000, 19 (23) :6299-6310
[2]   Use of the cell wall precursor lipid II by a pore-forming peptide antibiotic [J].
Breukink, E ;
Wiedemann, I ;
van Kraaij, C ;
Kuipers, OP ;
Sahl, HG ;
de Kruijff, B .
SCIENCE, 1999, 286 (5448) :2361-2364
[3]   MODE OF ACTION OF THE LANTIBIOTIC MERSACIDIN - INHIBITION OF PEPTIDOGLYCAN BIOSYNTHESIS VIA A NOVEL MECHANISM [J].
BROTZ, H ;
BIERBAUM, G ;
MARKUS, A ;
MOLITOR, E ;
SAHL, HG .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (03) :714-719
[4]   Role of lipid-bound peptidoglycan precursors in the formation of pores by nisin, epidermin and other lantibiotics [J].
Brötz, H ;
Josten, M ;
Wiedemann, I ;
Schneider, U ;
Götz, F ;
Bierbaum, G ;
Sahl, HG .
MOLECULAR MICROBIOLOGY, 1998, 30 (02) :317-327
[5]   The lantibiotic mersacidin inhibits peptidoglycan biosynthesis at the level of transglycosylation [J].
Brotz, H ;
Bierbaum, G ;
Reynolds, PE ;
Sahl, HG .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 246 (01) :193-199
[6]  
Burrage S, 2000, CHEM-EUR J, V6, P1455, DOI 10.1002/(SICI)1521-3765(20000417)6:8<1455::AID-CHEM1455>3.0.CO
[7]  
2-M
[8]   Biosynthesis and mode of action of lantibiotics [J].
Chatterjee, C ;
Paul, M ;
Xie, LL ;
van der Donk, WA .
CHEMICAL REVIEWS, 2005, 105 (02) :633-683
[9]   Lacticin 481 synthetase phosphorylates its substrate during lantibiotic production [J].
Chatterjee, C ;
Miller, LM ;
Leung, YL ;
Xie, LL ;
Yi, MS ;
Kelleher, NL ;
van der Donk, WA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (44) :15332-15333
[10]   Engineering dehydro amino acids and thioethers into peptides using lacticin 481 synthetase [J].
Chatterjee, Champak ;
Patton, Gregory C. ;
Cooper, Lisa ;
Paul, Moushumi ;
van der Donk, Wilfred A. .
CHEMISTRY & BIOLOGY, 2006, 13 (10) :1109-1117
12345678