IN VITRO STUDIES OF LANTIBIOTIC BIOSYNTHESIS

被引：23

作者：

Li, Bo ^{[1
,2
,3
]}

Cooper, Lisa E. ^{[1
,2
,3
]}

van der Donk, Wilfred A. ^{[1
,2
,3
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

[3] Univ Illinois, Howard Hughes Med Inst, Urbana, IL 61801 USA

来源：

COMPLEX ENZYMES IN MICROBIAL NATURAL PRODUCT BIOSYNTHESIS, PART A: OVERVIEW ARTICLES AND PEPTIDES | 2009年 / 458卷

关键词：

SITE-DIRECTED MUTAGENESIS; AMINO-ACID SUBSTITUTIONS; PRECURSOR LIPID II; LACTICIN-481; SYNTHETASE; LEADER PEPTIDE; POSTTRANSLATIONAL MODIFICATION; PEPTIDOGLYCAN BIOSYNTHESIS; OXIDATIVE DECARBOXYLATION; SUBSTRATE-SPECIFICITY; NISIN BIOSYNTHESIS;

D O I：

10.1016/S0076-6879(09)04821-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The lantibiotics are ribosomally synthesized and posttranslationally modified peptide antibiotics containing the thioether crosslinks lanthionine (Lan) and 3-methyllanthionine (MeLan) and typically also the dehydroamino acids dehydroalanine (Dha) and (Z)-dehydrobutyrine (Dhb). These modifications are formed by dehydration of Ser/Thr residues to produce the Dha and Dhb structures, and subsequent conjugate additions of Cys residues onto the unsaturated amino acids to form thioether rings (Lan and MeLan). Several of the enzymatic reactions involved in lantibiotic biosynthesis have been reconstituted in vitro in recent years and these systems as well as a general overview of lantibiotic biosynthesis are discussed in this chapter.

引用

页码：533 / +

页数：27

共 74 条

[71] On the regioselectivity of thioether formation by lacticin 481 synthetase [J].

Zhang, Xingang ;

Ni, Weijuan ;

van der Donk, Wilfred A. .

ORGANIC LETTERS, 2007, 9 (17) :3343-3346

[72] On the substrate specificity of dehydration by lacticin 481 synthetase [J].

Zhang, Xingang ;

van der Donk, Wilfred A. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (08) :2212-+

[73] Biomimetic stereoselective formation of methyllanthionine [J].

Zhou, H ;

van der Donk, WA .

ORGANIC LETTERS, 2002, 4 (08) :1335-1338

[74] Biomimetic studies on the mechanism of stereoselective lanthionine formation [J].

Zhu, YT ;

Gieselman, MD ;

Zhou, H ;

Averin, O ;

van der Donk, WA .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2003, 1 (19) :3304-3315

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