Rapid biological agent identification by surface-enhanced Raman spectroscopy

被引：13

作者：

Farquharson, S ^{[1
]}

Smith, WW ^{[1
]}

Elliott, S ^{[1
]}

Sperry, JF ^{[1
]}

机构：

[1] Adv Fuel Res, E Hartford, CT 06108 USA

来源：

AIR MONITORING AND DETECTION OF CHEMICAL AND BIOLOGICAL AGENTS II | 1999年 / 3855卷

关键词：

bioagent detection; SERS; DNA analysis; bacterial analysis; Raman spectroscopy;

D O I：

10.1117/12.371269

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The Chemical Weapons Convention prohibits the development, production, stockpiling, and use of warfare agents (chemical and biological), and requires their destruction. Yet their use persists and has been included in the terrorist's arsenal. Currently a number of analytical methods are being developed to perform rapid measurements of trace agents to ensure treaty compliance, as well as safe environments for military personal and the public at large. We have been investigating the ability of surface-enhanced Raman spectroscopy to detect bacterial nucleic acid-base pairs with sufficient sensitivity and selectivity to eliminate the need for enumeration used in polymerase chain reactions and culture growth, required by other measurement techniques. The design of a small volume, fiber optic coupled, electrolytic sample cell is presented along with analysis of DNA and RNA separated from non-toxic bacteria.

引用

页码：110 / 116

页数：7

共 30 条

[1]

Connes J., 1961, Rev. Opt, V40, P45

[2] SURFACE ENHANCED RAMAN-SPECTRA OF NUCLEIC-ACID COMPONENTS ADSORBED AT A SILVER ELECTRODE [J].