Complete nucleotide sequence and organization of the naphthalene catabolic plasmid pND6-1 from Pseudomonas sp strain ND6

被引:83
作者
Li, W
Shi, JD
Wang, XG
Han, YN
Tong, W
Ma, L
Liu, B [1 ]
Cai, BL
机构
[1] Chinese Acad Sci, Beijing Genom Inst, Beijing 101300, Peoples R China
[2] Nankai Univ, Dept Microbiol, Tianjin 300071, Peoples R China
基金
中国国家自然科学基金;
关键词
naphthalene catabolic plasmid; sequencing; genome annotation; comparative genomics;
D O I
10.1016/j.gene.2004.03.027
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Pseudomonas sp. strain ND6, which was isolated from industrial wastewater in Tianjin, China, was capable of dissimilating naphthalene as sole carbon and energy sources. We identified one plasmid, pND6-1, which was associated with the metabolism of naphthalene and determined the complete nucleotide sequence of pND6-1 (101,858 bp) using a whole-genome-shotgun approach. Computational analyses indicated that the naphthalene metabolism of the strain ND6 is associated with this plasmid. This is the first report of a complete sequence of naphthalene catabolic plasmid. pND6-1 encodes 102 putative coding sequences (CDSs). Among them, 23 CDSs were predicted to be involved in naphthalene catabolism, 14 were predicted to be involved in transposition and integration, 2 encoded putative transporters, 3 were putative transcriptional regulators, and 9 were proteins necessary for plasmid replication and partitioning. Most of the naphthalene catabolic genes of pND6-1 have 99-100% identity in amino acid sequences homologous to their nearest counterparts found in plasmid pDTG1, NAH7 and in a chromosome region in Pseudomonas stutzeri AN10 except for two duplicated genes (ND013 and ND016). Results of this study indicated that globally distributed naphthalene catabolic genes are highly conserved among different bacterial species. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:231 / 240
页数:10
相关论文
共 35 条
[31]  
Williams Peter A., 1994, Biodegradation, V5, P195, DOI 10.1007/BF00696460
[32]  
Wyndham R. Campbell, 1994, Biodegradation, V5, P323, DOI 10.1007/BF00696468
[33]   GENETICS OF NAPHTHALENE CATABOLISM IN PSEUDOMONADS [J].
YEN, KM ;
SERDAR, CM .
CRC CRITICAL REVIEWS IN MICROBIOLOGY, 1988, 15 (03) :247-268
[34]  
ZHANG X, 2000, CHIN J APPL ENV BIOL, V6, P187
[35]   nag genes of Ralstonia (formerly Pseudomonas) sp strain U2 encoding enzymes for gentisate catabolism [J].
Zhou, NY ;
Fuenmayor, SL ;
Williams, PA .
JOURNAL OF BACTERIOLOGY, 2001, 183 (02) :700-708