Development of an electrochemical immunoassay for rapid detection of E. coli using anodic stripping voltammetry based on Cu@Au nanoparticles as antibody labels

被引：49

作者：

Zhang, Xinai ^{[1
]}

Geng, Ping ^{[1
]}

Liu, Huijie ^{[1
]}

Teng, Yingqiao ^{[1
]}

Liu, Yajun ^{[1
]}

Wang, Qingjiang ^{[1
]}

Zhang, Wen ^{[1
]}

Jin, Litong ^{[1
]}

Jiang, Lai ^{[2
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

[2] Shanghai Jiao Tong Univ, Dept Surg, Intens Care Unit, Xinhua Hosp,Coll Med, Shanghai 200092, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2009年 / 24卷 / 07期

关键词：

E; coli; Cu@Au nanoparticles; Anodic stripping voltammetry; Electrochemical immunoassay; COLLOIDAL GOLD LABEL; ESCHERICHIA-COLI; DRINKING-WATER; ASSAY; COLIFORMS; ENZYME; IMMUNOSENSOR; SPECTROSCOPY; INTEGRATION; BIOSENSOR;

D O I：

10.1016/j.bios.2008.11.019

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A sensitive electrochemical immunoassay for rapid detection of Escherichia coli has been developed by anodic stripping voltammetry (ASV) based on core-shell Cu@Au nanoparticles (NPs) as anti-E. coli antibody labels. The characteristics of Cu@Au NPs before and after binding with antibody were confirmed by transmission electron microscopy (TEM). After Cu@Au-labeled antibody reacted with the immobilized E. coli on Polystyrene (PS)-modified ITO chip, Cu@Au NPs were dissolved by oxidation to the metal ionic forms, and the released Cul(2+) ions were determined at GC/Nafion/Hg modified electrode by ASV. The utilization of GC/Nafion/Hg modified electrode could enhance the sensitivity for Cu2+ detection with a concentration as low as 9.0 x 10(-12) mol/L Since Cu@Au NPs labels were only present when antibody reacted with E. coli, the amount of Cu2+ directly reflected the number of E coli. The technique could detect E. coli with a detection limit of 30CFU/mL and the overall analysis could be completed in 2 h. By introducing a pre-enrichment step, a concentration of 3 CFU/10 mL E. coli in surface water was detected by the electrochemical immunoassay. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：2155 / 2159

页数：5