Novel human papillomavirus type 18 replicon and its application in screening the antiviral effects of cytokines

被引：8

作者：

Satsuka, Ayano ^{[1
,2
]}

Yoshida, Satoshi ^{[1
,3
]}

Kajitani, Naoko ^{[1
,2
]}

Nakamura, Hiroyasu ^{[1
,3
]}

Sakai, Hiroyuki ^{[1
]}

机构：

[1] Kyoto Univ, Inst Virus Res, Dept Viral Oncol, Lab Gene Anal, Kyoto 606, Japan

[2] Kyoto Univ, Lab Mammalian Mol Biol, Grad Sch Biosci, Kyoto 606, Japan

[3] Kyoto Univ, Dept Viral Oncol, Grad Sch Med Sci, Kyoto 606, Japan

来源：

CANCER SCIENCE | 2010年 / 101卷 / 02期

基金：

日本学术振兴会;

关键词：

TUMOR-NECROSIS-FACTOR; PRIMARY HUMAN KERATINOCYTES; VIRAL LIFE-CYCLE; CELL-LINE; EPITHELIAL DIFFERENTIATION; CERVICAL-CARCINOMA; GENE-EXPRESSION; DNA-REPLICATION; E7; IMMORTALIZATION;

D O I：

10.1111/j.1349-7006.2009.01411.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Human papillomaviruses (HPVs) infect the stratified epithelial organ. The infection induces benign tumors, which occasionally progress into malignant tumors. To elucidate the virus-induced tumorigenesis, an understanding of the lifecycle of HPV is crucial. In this report, we developed a new system for the analysis of the HPV lifecycle. The new system consists of a novel HPV replicon and an organotypic "raft'' culture, by which the HPV-DNA is maintained stably in normal human keratinocytes for a long period and the viral vegetative replication is reproduced. This system will benefit biochemical and genetic studies on the lifecycle of HPV and tumorigenesis. This system is also valuable in screening for antiviral compounds. We confirmed its usefulness by evaluating the antivirus effect of cytokines. (Cancer Sci 2010; 101: 536-542)

引用

页码：536 / 542

页数：7

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