Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures

被引:88
作者
Bakermans, Corien
Tollaksen, Sandra L.
Giometti, Carol S.
Wilkerson, Curtis
Tiedje, James M.
Thomashow, Michael F.
机构
[1] Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA
[2] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA
[3] Michigan State Univ, Res Technol Support Facil, E Lansing, MI 48824 USA
关键词
psychrophiles; proteome; low-temperature; psychrobacter; cold acclimation;
D O I
10.1007/s00792-006-0042-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is crucial to examine the physiological processes of psychrophiles at temperatures below 4 degrees C, particularly to facilitate extrapolation of laboratory results to in situ activity. Using two dimensional electrophoresis, we examined patterns of protein abundance during growth at 16, 4, and -4 degrees C of the eurypsychrophile Psychrobacter cryohalolentis K5 and report the first identification of cold inducible proteins (CIPs) present during growth at subzero temperatures. Growth temperature substantially reprogrammed the proteome; the relative abundance of 303 of the 618 protein spots detected (similar to 31% of the proteins at each growth temperature) varied significantly with temperature. Five CIPs were detected specifically at -4 inverted perpendicular degrees C; their identities (AtpF, EF-Ts, TolC, Pcryo_1988, and FecA) suggested specific stress on energy production, protein synthesis, and transport during growth at subzero temperatures. The need for continual relief of low-temperature stress on these cellular processes was confirmed via identification of 22 additional CIPs whose abundance increased during growth at -4 degrees C (relative to higher temperatures). Our data suggested that iron may be limiting during growth at subzero temperatures and that a cold-adapted allele was employed at -4 degrees C for transport of iron. In summary, these data suggest that low-temperature stresses continue to intensify as growth temperatures decrease to -4 degrees C.
引用
收藏
页码:343 / 354
页数:12
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