Comparison of Optical Bioluminescence Reporter Gene and Superparamagnetic Iron Oxide MR Contrast Agent as Cell Markers for Noninvasive Imaging of Cardiac Cell Transplantation

被引：76

作者：

Chen, Ian Y. ^{[1
,2
,4
,5
]}

Greve, Joan M. ^{[5
]}

Gheysens, Olivier ^{[1
,2
,4
,5
]}

Willmann, Juergen K. ^{[1
,2
,4
,5
]}

Rodriguez-Porcel, Martin ^{[1
,2
,4
,5
]}

Chu, Pauline

Sheikh, Ahmad Y. ^{[1
,3
]}

Faranesh, Anthony Z. ^{[1
,4
]}

Paulmurugan, Ramasamy ^{[1
,2
,4
,5
]}

Yang, Phillip C. ^{[6
]}

Wu, Joseph C. ^{[1
,4
,6
]}

Gambhir, Sanjiv S. ^{[1
,2
,4
,5
]}

机构：

[1] Stanford Univ, Sch Med, MIPS, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, BioX Program, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Dept Cardiothorac, Stanford, CA 94305 USA

[4] Stanford Univ, Sch Med, Dept Radiol, Stanford, CA 94305 USA

[5] Stanford Univ, Sch Med, Dept Bioengn, Stanford, CA 94305 USA

[6] Stanford Univ, Sch Med, Dept Med, Div Cardiovasc Med, Stanford, CA 94305 USA

来源：

MOLECULAR IMAGING AND BIOLOGY | 2009年 / 11卷 / 03期

关键词：

Optical bioluminescence imaging; Magnetic resonance imaging; Reporter gene; Contrast agent; Cell marker; Cell transplantation; MESENCHYMAL STEM-CELLS; SURVIVAL; CARDIOMYOCYTES; THERAPY;

D O I：

10.1007/s11307-008-0182-z

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

100231 [临床病理学]; 100902 [航空航天医学];

摘要：

In this study, we compared firefly luciferase (Fluc) reporter gene and superparamagnetic iron oxide (Feridex) as cell markers for longitudinal monitoring of cardiomyoblast graft survival using optical bioluminescence imaging (BLI) and magnetic resonance imaging (MRI), respectively. Rats (n = 31) underwent an intramyocardial injection of cardiomyoblasts (2 x 10(6)) labeled with Fluc, Feridex, or no marker (control) or an injection of Feridex alone (75 mu g). Afterward, rats were serially imaged with BLI or MRI and killed at different time points for histological analysis. BLI revealed a drastically different cell survival kinetics (half-life = 2.65 days over 6 days) than that revealed by MRI (half-life = 16.8 days over 80 days). Injection of Feridex alone led to prolonged tissue retention of Feridex (a parts per thousand yen16 days) and persistent MR signal (a parts per thousand yen42 days). Fluc BLI reporter gene imaging is a more accurate gauge of transplanted cell survival as compared to MRI of Feridex-labeled cells.

引用

页码：178 / 187

页数：10

共 33 条

[1]

Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging [J].