Localization of a calcium sensitive binding site for gelsolin on actin subdomain 1: Implication for severing process

被引：5

作者：

Feinberg, J ^{[1
]}

Lebart, MC ^{[1
]}

Benyamin, Y ^{[1
]}

Roustan, C ^{[1
]}

机构：

[1] UNIV MONTPELLIER 1,CTR RECH BIOCHIM MACROMOL,ECOLE PRAT HAUTES ETUD,CNRS,F-34033 MONTPELLIER,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 233卷 / 01期

关键词：

D O I：

10.1006/bbrc.1997.6402

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding of the N-terminal domain (S1) of gelsolin to monomeric actin has been extensively documented. In contrast, the location of the C-terminal calcium dependent domains (S4-6) interacting with the actin filament during the severing process remains uncertain, In this study, we have identified a new interface that supports calcium dependent gelsolin binding to actin. This site is located in a critical position towards actin-actin contact in the filament and in the vicinity of the phalloidin site. Using specific antibody and synthetic peptides derived from actin sequence within 105-132 residues, this interface was finally ascribed to the segment 112-120 on the actin subdomain-1. (C) 1997 Academic Press.

引用

页码：61 / 65

页数：5

共 26 条

[1]

ALLEN PG, 1994, J BIOL CHEM, V269, P32916

[2] GELSOLIN HAS 3 ACTIN-BINDING SITES [J].