Fast genomic μChIP-chip from 1,000 cells

被引：30

作者：

Dahl, John Arne ^{[1
]}

Reiner, Andrew H. ^{[1
]}

Collas, Philippe ^{[1
]}

机构：

[1] Univ Oslo, Fac Med, Dept Biochem, Inst Basic Med Sci, N-0317 Oslo, Norway

来源：

GENOME BIOLOGY | 2009年 / 10卷 / 02期

关键词：

CHROMATIN IMMUNOPRECIPITATION ASSAY; EMBRYONIC STEM-CELLS; DNA; PLURIPOTENCY; FORMALDEHYDE; NETWORK; GENES;

D O I：

10.1186/gb-2009-10-2-r13

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (mu ChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. mu ChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.

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页数：11

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