Efforts toward the expansion of the genetic alphabet: Information storage and replication with unnatural hydrophobic base pairs

被引：180

作者：

Ogawa, AK ^{[1
]}

Wu, YQ ^{[1
]}

McMinn, DL ^{[1
]}

Liu, JQ ^{[1
]}

Schultz, PG ^{[1
]}

Romesberg, FE ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2000年 / 122卷 / 14期

关键词：

D O I：

10.1021/ja9940064

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The faithful recognition of the interstrand hydrogen bonds between complementary nucleobases forms the foundation of the genetic code. The ability to replicate DNA containing a stable third base pair would allow for an expansion of the information content of DNA by supplementing the existing two base pairs of the genetic alphabet with a third. We report the optimization of unnatural nucleobases whose pairing in duplex DNA is based on interbase hydrophobic interactions. We show that the stability and selectivity of such unnatural base pairs may be comparable to, or even exceed, that of native pairs. We also demonstrate that several unnatural base pairs are incorporated into DNA by Klenow fragment of Escherichia coli DNA polymerase I with an efficiency equivalent to that of native DNA synthesis. Moreover, the unnatural bases are orthogonal to the native bases, with correct pairing being favored by at least an order of magnitude relative to mispairing.

引用

页码：3274 / 3287

页数：14

共 35 条

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