Characterizing potential bacterial biocontrol agents for suppression of Rhizobium vitis, causal agent of crown gall disease in grapevines

被引:23
作者
Eastwell, Kenneth C. [1 ]
Sholberg, Peter L.
Sayler, Ronald J.
机构
[1] Washington State Univ, Dept Plant Pathol, IAREC, Prosser, WA 99350 USA
[2] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Summerland, BC V0H 1Z0, Canada
[3] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA
关键词
crown gall; biocontrol; endophytic bacteria; Vitis vinifera; Agrobacterium vitis;
D O I
10.1016/j.cropro.2006.03.004
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Several bacteria were evaluated in greenhouse studies for their effectiveness at preventing formation of galls induced by Rhizobium vitis. the Gram negative bacterium that causes crown gall disease of grapevines. Three endophytic bacteria isolated from Malus domestica yielded promising results and were selected for further examination: Pseudomonas fluorescens isolate 1100-6, Bacillus subtilis isolate EN63-1. and Bacillus species isolate EN71-1. In planta experiments were initially performed on Nicotiana glauca to facilitate measurement of gall development. The effect of the interval between inoculation of N. glauca with the biocontrol agent and exposure to R. vitis was evaluated. All three bacteria significantly reduced gall size when applied 25 or 86 days before inoculation with R. vitis, and reduced the population of R. vitis relative to control plants. P. fluorescens '1100-6' also reduced the percentage of R. vitis inoculations that yielded galls. P. fluorescens '1100-6' was genetically tagged to allow exploration of its localization in grapevines. In growth chamber studies, P. fluorescens '1100-6' was found to survive in the rhizoplane of grapevines for 6 months. Microscopic examination showed that the genetically modified P. fluorescens '1100-6' predominantly occupied xylem and pith tissues, and occasionally persisted on external surfaces of grapevines. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1191 / 1200
页数:10
相关论文
共 49 条
[11]   Characterization of Agrobacterium vitis strains isolated from feral Vitis riparia [J].
Burr, TJ ;
Reid, CL ;
Adams, CE ;
Momol, EA .
PLANT DISEASE, 1999, 83 (02) :102-107
[12]   Biological control of grape crown gall by strain F2/5 is not associated with agrocin production or competition for attachment sites on grape cells [J].
Burr, TJ ;
Reid, CL ;
Tagliati, E ;
Bazzi, C ;
Sule, S .
PHYTOPATHOLOGY, 1997, 87 (07) :706-711
[13]  
BURR TJ, 1994, AM J ENOL VITICULT, V45, P213
[14]   Seasonal variation in pH, carbohydrate and nitrogen of xylem exudate of Vitis vinifera [J].
Campbell, JA ;
Strother, S .
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1996, 23 (01) :115-118
[15]  
Citterio B, 2004, EUR J HISTOCHEM, V48, P191
[16]   Altered srf expression in Bacillus subtilis resulting from changes in culture pH is dependent on the Spo0K oligopeptide permease and the ComQX system of extracellular control [J].
Cosby, WM ;
Vollenbroich, D ;
Lee, OH ;
Zuber, P .
JOURNAL OF BACTERIOLOGY, 1998, 180 (06) :1438-1445
[17]   Biological control of apple blue mold with Pseudomonas fluorescens [J].
Etebarian, HR ;
Sholberg, PL ;
Eastwell, KC ;
Sayler, RJ .
CANADIAN JOURNAL OF MICROBIOLOGY, 2005, 51 (07) :591-598
[18]   A rapid polymerase chain reaction-based assay characterizing rhizosphere populations of 2,4-diacetylphloroglucinol-producing bacteria [J].
Gardener, BBM ;
Mavrodi, DV ;
Thomashow, LS ;
Weller, DM .
PHYTOPATHOLOGY, 2001, 91 (01) :44-54
[19]   Modeling the rate of attachment of Listeria monocytogenes, Pantoea agglomerans, and Pseudomonas fluorescens to, and the probability of their detachment from, potato tissue at 10°C [J].
Garrood, MJ ;
Wilson, PDG ;
Brocklehurst, TF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (06) :3558-3565
[20]   Expression of a crown gall biological control phenotype in an avirulent strain of Agrobacterium vitis by addition of the trifolitoxin production and resistance genes [J].
Herlache T.C. ;
Triplett E.W. .
BMC Biotechnology, 2 (1)