Recent advances in fluorescent probes for the detection of reactive oxygen species

被引:286
作者
Soh, Nobuaki [1 ]
机构
[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
关键词
fluorescent probe; reactive oxygen species (ROS); singlet oxygen (O-1(2)); hydrogen peroxide (H2O2); hydroxyl radical ((OH)-O-.); superoxide anion (O-2(-.));
D O I
10.1007/s00216-006-0366-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Reactive oxygen species (ROS) have captured the interest of many researchers in the chemical, biological, and medical fields since they are thought to be associated with various pathological conditions. Fluorescent probes for the detection of ROS are promising tools with which to enhance our understanding of the physiological roles of ROS, because they provide spatial and temporal information about target biomolecules in in vivo cellular systems. ROS probes, designed to detect specific ROS with a high selectivity, would be desirable, since it is now becoming clear that each ROS has its own unique physiological activity. However, dihydro-compounds such as 2',7'-dichlorodihydrofluorescein (DCFH), which have traditionally been used for detecting ROS, tend to react with a wide variety of ROS and are not completely photostable. Some attractive fluorescent probes that exhibit a high degree of selectivity toward specific ROS have recently been reported, and these selective probes are expected to have great potential for elucidating unknown physiological mechanisms associated with their target ROS. This review focuses on the design, detection mechanism, and performance of fluorescent probes for the detection of singlet oxygen (O-1(2)), hydrogen peroxide (H2O2), hydroxyl radicals ((OH)-O-.), or superoxide anion (O-2(-.)), a field in which remarkable progress has been achieved in the last few years.
引用
收藏
页码:532 / 543
页数:12
相关论文
共 97 条
[1]   Method to overcome photoreaction, a serious drawback to the use of dichlorofluorescin in evaluation of reactive oxygen species [J].
Afzal, M ;
Matsugo, S ;
Sasai, M ;
Xu, BH ;
Aoyama, K ;
Takeuchi, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 304 (04) :619-624
[2]  
AKASAKA K, 1987, ANAL LETT, V20, P731
[3]   NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH A FLUOROMETRIC POSTCOLUMN DETECTION SYSTEM FOR LIPID HYDROPEROXIDES [J].
AKASAKA, K ;
OHRUI, H ;
MEGURO, H .
JOURNAL OF CHROMATOGRAPHY, 1993, 628 (01) :31-35
[4]   Intracellular generation of reactive oxygen species during nonhypoxic lung ischemia [J].
AlMehdi, AB ;
Shuman, H ;
Fisher, AB .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 1997, 272 (02) :L294-L300
[5]   Critical evaluation of the use of hydroethidine as a measure of superoxide anion radical [J].
Benov, L ;
Sztejnberg, L ;
Fridovich, I .
FREE RADICAL BIOLOGY AND MEDICINE, 1998, 25 (07) :826-831
[6]   CHEMICALLY MEDIATED FLUORESCENCE YIELD SWITCHING IN NITROXIDE FLUOROPHORE ADDUCTS - OPTICAL SENSORS OF RADICAL REDOX REACTIONS [J].
BLOUGH, NV ;
SIMPSON, DJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (06) :1915-1917
[7]   Glutathione propagates oxidative stress triggered by Myeloperoxidase in HL-60 cells - Evidence for glutathionyl radical-induced peroxidation of phospholipids and cytotoxicity [J].
Borisenko, GG ;
Martin, I ;
Zhao, Q ;
Amoscato, AA ;
Tyurina, YY ;
Kagan, VE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (22) :23453-23462
[8]   A selective, cell-permeable optical probe for hydrogen peroxide in living cells [J].
Chang, MCY ;
Pralle, A ;
Isacoff, EY ;
Chang, CJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (47) :15392-15393
[9]   Redox-dependent trafficking of 2,3,4,5,6-pentafluorodihydrotetramethylrosaimine, a novel fluorogenic indicator of cellular oxidative activity [J].
Chen, SR ;
Gee, KR .
FREE RADICAL BIOLOGY AND MEDICINE, 2000, 28 (08) :1266-1278
[10]   Redox control of signal transduction, gene expression and cellular senescence [J].
Esposito, F ;
Ammendola, R ;
Faraonio, R ;
Russo, T ;
Cimino, F .
NEUROCHEMICAL RESEARCH, 2004, 29 (03) :617-628