Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

被引：176

作者：

Rappaz, Benjamin ^{[1
]}

Cano, Elena ^{[2
]}

Colomb, Tristan ^{[3
]}

Kuehn, Jonas ^{[4
]}

Depeursinge, Christian ^{[4
]}

Simanis, Viesturs ^{[2
]}

Magistretti, Pierre J.

Marquet, Pierre ^{[5
]}

机构：

[1] Ecole Polytech Fed Lausanne, LNDC BMI SV, Stn 15, Brain Mind Inst, CH-1015 Lausanne, VD, Switzerland

[2] Ecole Polytech Fed Lausanne, ISREC, CH-1015 Lausanne, Switzerland

[3] Lyncee Tec SA, PSE A, CH-1015 Lausanne, Switzerland

[4] Ecole Polytech Fed Lausanne, Imaging & Appl Opt Inst, CH-1015 Lausanne, Switzerland

[5] DP CHUV, Dept Psychiat, Ctr Neurosci Psychiat, CH-1008 Prilly, Switzerland

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2009年 / 14卷 / 03期

基金：

瑞士国家科学基金会;

关键词：

digital holographic microscopy; quantitative phase image; dry mass; cell cycle; cell growth; fission yeast; SCHIZOSACCHAROMYCES-POMBE; DIVISION CYCLE; PHASE MICROSCOPY; GENETIC-CONTROL; LIVING CELLS; DYNAMICS; CONTRAST; GROWTH;

D O I：

10.1117/1.3147385

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3147385]

引用

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