Meiotic contraction of CAG repeats in Saccharomyces cerevisiae

被引：4

作者：

Arai, N ^{[1
]}

Akiyama, R ^{[1
]}

Niimi, N ^{[1
]}

Nakatsubo, H ^{[1
]}

Inoue, T ^{[1
]}

机构：

[1] Nihon Univ, Coll Bioresource Sci, Dept Appl Biol Sci, Lab Nucl Acid Sci, Fujisawa, Kanagawa 2520813, Japan

来源：

GENES & GENETIC SYSTEMS | 1999年 / 74卷 / 04期

关键词：

D O I：

10.1266/ggs.74.159

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several human neurodegenerative disorders are caused by expansion of CAG repeats that occurs during meiosis or gametogenesis. We anticipated that the CAG repeats cloned in a plasmid of Saccharomyces cerevisiae might undergo a change in the number of repeats during meiosis and sporulation. To test this possibility, we devised a new method to change in vitro the number of CAG repeats and constructed plasmids carrying (CAG)(39), (CAG)(65) or (CAG)(123) from a plasmid carrying (CAG)(18) We monitored the number of colonies showing an altered length of the repeat tracts during mitosis and meiotic growth. Contraction of long GAG; repeat was found to occur frequently, whereas a few cases of expansion were observed. The contraction was equally enhanced in both orientations when the host cells grew through meiosis. Thus, our results suggest that long CAG repeats are destabilized during meiosis or gametogenesis in S. cerevisiae.

引用

页码：159 / 167

页数：9

共 29 条

[11] Mitotic and meiotic instability of the CAG trinucleotide repeat in spinocerebellar ataxia type 1 [J].

Koefoed, P ;

Hasholt, L ;

Fenger, K ;

Nielsen, JE ;

Eiberg, H ;

Buschard, K ;

Sorensen, SA .

HUMAN GENETICS, 1998, 103 (05) :564-569

[12] UNSTABLE EXPANSION OF CAG REPEAT IN HEREDITARY DENTATORUBRAL-PALLIDOLUYSIAN ATROPHY (DRPLA) [J].

KOIDE, R ;

IKEUCHI, T ;

ONODERA, O ;

TANAKA, H ;

IGARASHI, S ;

ENDO, K ;

TAKAHASHI, H ;

KONDO, R ;

ISHIKAWA, A ;

HAYASHI, T ;

SAITO, M ;

TOMODA, A ;

MIIKE, T ;

NAITO, H ;

IKUTA, F ;

TSUJI, S .

NATURE GENETICS, 1994, 6 (01) :9-13

[13]

LA SA, 1991, NATURE, V352, P77

[14]

LEEFLANG EP, 1995, HUM MOL GENET, V4, P1519

[15] A NOVEL GENE CONTAINING A TRINUCLEOTIDE REPEAT THAT IS EXPANDED AND UNSTABLE ON HUNTINGTONS-DISEASE CHROMOSOMES [J].

MACDONALD, ME ;

AMBROSE, CM ;

DUYAO, MP ;

MYERS, RH ;

LIN, C ;

SRINIDHI, L ;

BARNES, G ;

TAYLOR, SA ;

JAMES, M ;

GROOT, N ;

MACFARLANE, H ;

JENKINS, B ;

ANDERSON, MA ;

WEXLER, NS ;

GUSELLA, JF ;

BATES, GP ;

BAXENDALE, S ;

HUMMERICH, H ;

KIRBY, S ;

NORTH, M ;

YOUNGMAN, S ;

MOTT, R ;

ZEHETNER, G ;

SEDLACEK, Z ;

POUSTKA, A ;

FRISCHAUF, AM ;

LEHRACH, H ;

BUCKLER, AJ ;

CHURCH, D ;

DOUCETTESTAMM, L ;

ODONOVAN, MC ;

RIBARAMIREZ, L ;

SHAH, M ;

STANTON, VP ;

STROBEL, SA ;

DRATHS, KM ;

WALES, JL ;

DERVAN, P ;

HOUSMAN, DE ;

ALTHERR, M ;

SHIANG, R ;

THOMPSON, L ;

FIELDER, T ;

WASMUTH, JJ ;

TAGLE, D ;

VALDES, J ;

ELMER, L ;

ALLARD, M ;

CASTILLA, L ;

SWAROOP, M .

CELL, 1993, 72 (06) :971-983

[16] MYOTONIC-DYSTROPHY MUTATION - AN UNSTABLE CTG REPEAT IN THE 3' UNTRANSLATED REGION OF THE GENE [J].

MAHADEVAN, M ;

TSILFIDIS, C ;

SABOURIN, L ;

SHUTLER, G ;

AMEMIYA, C ;

JANSEN, G ;

NEVILLE, C ;

NARANG, M ;

BARCELO, J ;

OHOY, K ;

LEBLOND, S ;

EARLEMACDONALD, J ;

DEJONG, PJ ;

WIERINGA, B ;

KORNELUK, RG .

SCIENCE, 1992, 255 (5049) :1253-1255

[17]

Maurer DJ, 1996, MOL CELL BIOL, V16, P6617

[18] Orientation-dependent and sequence-specific expansions of CTG/CAG trinucleotide repeats in Saccharomyces cerevisiae [J].

Miret, JJ ;

Pessoa-Brandao, L ;

Lahue, RS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12438-12443

[19] Instability of CAG and CTG trinucleotide repeats in Saccharomyces cerevisiae [J].

Miret, JJ ;

PessoaBrandao, L ;

Lahue, RS .

MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (06) :3382-3387

[20] DENTATORUBRAL AND PALLIDOLUYSIAN ATROPHY EXPANSION OF AN UNSTABLE CAG TRINUCLEOTIDE ON CHROMOSOME-12P [J].

NAGAFUCHI, S ;

YANAGISAWA, H ;

SATO, K ;

SHIRAYAMA, T ;

OHSAKI, E ;

BUNDO, M ;

TAKEDA, T ;

TADOKORO, K ;

KONDO, I ;

MURAYAMA, N ;

TANAKA, Y ;

KIKUSHIMA, H ;

UMINO, K ;

KUROSAWA, H ;

FURUKAWA, T ;

NIHEI, K ;

INOUE, T ;

SANO, A ;

KOMURE, O ;

TAKAHASHI, M ;

YOSHIZAWA, T ;

KANAZAWA, I ;

YAMADA, M .

NATURE GENETICS, 1994, 6 (01) :14-18

← 1 2 3 →