Protein purification by ultrafiltration with pre-treated membrane

被引：100

作者：

Ghosh, R ^{[1
]}

Cui, ZF ^{[1
]}

机构：

[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

来源：

JOURNAL OF MEMBRANE SCIENCE | 2000年 / 167卷 / 01期

关键词：

membrane pre-treatment; ultrafiltration; lysozyme; purification; egg white;

D O I：

10.1016/S0376-7388(99)00275-6

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The paper discusses separation of lysozyme from chicken egg white (CEW) by ultrafiltration using 50 kDa MWCO polysulfone membrane pre-treated with myoglobin. The transmission of lysozyme was about 26% higher with the pre-treated membrane than with the native membrane. The transmission of the other major CEW proteins, i.e., ovalbumin and conalbumin, were found to depend on the transmembrane pressure (TMP), transmission decreased with increase in TMP. Thus, using a combination of surface pre-treatment and TMP adjustment, conditions were obtained whereby lysozyme was totally transmitted while ovalbumin and other CEW proteins were almost totally rejected. At a TMP of 20 kPa, the percentage purity of lysozyme was about 18%, while at a TMP of 120 kPa, the purity was greater than 96%. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：47 / 53

页数：7

共 16 条

[1] 2-STEP CHROMATOGRAPHIC PROCEDURE FOR THE PURIFICATION OF HEN EGG-WHITE OVOMUCIN, LYSOZYME, OVOTRANSFERRIN AND OVALBUMIN AND CHARACTERIZATION OF PURIFIED PROTEINS [J].