Analysis of the expression of the Trichoderma harzianum ech42 gene in two isogenic clones of Escherichia coli by surface response methodology

被引：17

作者：

De León, A

Jiménez-Islas, H

González-Cuevas, M

de la Rosa, APB

机构：

[1] Inst Potosino Invest Cientif & Tecnol, Dept Mol Biol, San Luis Potosi 78231, SLP, Mexico

[2] Inst Tecnol Celaya, Dept Ingn Bioquim, Guanajuato 38010, Mexico

来源：

PROCESS BIOCHEMISTRY | 2004年 / 39卷 / 12期

关键词：

endochitinase; Trichoderma harzianum; surface response methodology; Escherichia coli;

D O I：

10.1016/j.procbio.2003.11.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The endochitinase gene ech42 from Trichoderma harzianum was cloned and expressed in Escherichia coli BL21(D3)pLysS using a pET-19b expression system. The effect of temperature, cell concentrations and inductor concentration on protein expression were evaluated by response surface methodology. Applying a second-order model to describe the amount of endochitinase produced by two selected clones, demonstrated that different optimal conditions of expression were found among clones, indicating that the best environmental conditions for expression among the several isogenic clones differed. (C) 2003 Elsevier Ltd. All rights reserved.

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收藏

页码：2173 / 2178

页数：6

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