Existence of a plant tyrosylprotein sulfotransferase:: novel plant enzyme catalyzing tyrosine O-sulfation of preprophytosulfokine variants in vitro

被引：50

作者：

Hanai, H ^{[1
]}

Nakayama, D ^{[1
]}

Yang, HP ^{[1
]}

Matsubayashi, Y ^{[1
]}

Hirota, Y ^{[1
]}

Sakagami, Y ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Lab Bioact Nat Prod Chem, Nagoya, Aichi 4648601, Japan

来源：

FEBS LETTERS | 2000年 / 470卷 / 02期

基金：

日本学术振兴会;

关键词：

phytosulfokine-alpha; tyrosine O-sulfation; tyrosylprotein sulfotransferase;

D O I：

10.1016/S0014-5793(00)01299-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An in vitro assay system to detect tyrosylprotein sulfotransferase (TPST) activity of higher plant cells was established, using synthetic oligopeptides based on the deduced amino acid sequence of a phytosulfokine-alpha (PSB-alpha) precursor, TPST activity nas found in microsomal membrane fractions of rice, asparagus and carrot cells and it was confirmed that acidic amino acid residues adjacent to the tyrosine residues of acceptor peptides were essential to the sulfation reaction. The asparagus TEST exhibited a broad pH optimum of 7.0-8.5, required manganese ions for maximal activity and appeared to be a membrane-bound protein localized in the Golgi apparatus. These enzymes should be defined as a new class of plant sulfotransferases that catalyze tyrosine O-sulfation of a PSK-alpha precursor and other unknown proteins. (C) 2000 Federation of European Biochemical Societies.

引用

页码：97 / 101

页数：5

共 26 条

[1] TYROSINE SULFATION IS A TRANS-GOLGI-SPECIFIC PROTEIN MODIFICATION [J].

BAEUERLE, PA ;

HUTTNER, WB .

JOURNAL OF CELL BIOLOGY, 1987, 105 (06) :2655-2664

[2] Existence of distinct tyrosylprotein sulfotransferase genes: Molecular characterization of tyrosylprotein sulfotransferase-2 [J].

Beisswanger, R ;

Corbeil, D ;

Vannier, C ;

Thiele, C ;

Dohrmann, U ;

Kellner, R ;

Ashman, K ;

Niehrs, C ;

Huttner, WB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (19) :11134-11139

[3]

Domiano S.R, 1989, J BIOL CHEM, V264, P899

[4]

HANAI H, 2000, IN PRESS PLANT CELL, V41

[5]

KAWASAKI S, 1990, SOSHIKIBAIYO, V16, P262

[6]

LEE RWH, 1983, J BIOL CHEM, V258, P1326

[7] Characterization of specific binding sites for a mitogenic sulfated peptide, phytosulfokine-α, in the plasma-membrane fraction derived from Oryza sativa L. [J].

Matsubayashi, Y ;

Sakagami, Y .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 262 (03) :666-671

[8] Phytosulfokine-alpha, a sulfated pentapeptide, stimulates the proliferation of rice cells by means of specific high-and low-affinity binding sites [J].

Matsubayashi, Y ;

Takagi, L ;

Sakagami, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (24) :13357-13362

[9] The endogenous sulfated pentapeptide phytosulfokine-α stimulates tracheary element differentiation of isolated mesophyll cells of zinnia [J].

Matsubayashi, Y ;

Takagi, L ;

Omura, N ;

Morita, A ;

Sakagami, Y .

PLANT PHYSIOLOGY, 1999, 120 (04) :1043-1048

[10] Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L [J].

Matsubayashi, Y ;

Sakagami, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (15) :7623-7627

← 1 2 3 →