How to become a uropathogen:: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains

被引:267
作者
Brzuszkiewicz, Elzbieta
Brueggemann, Holger
Liesegang, Heiko
Emmerth, Melanie
Oeschlaeger, Tobias
Nagy, Gabor
Albermann, Kaj
Wagner, Christian
Buchrieser, Carmen
Emody, Levente
Gottschalk, Gerhard
Hackert, Joerg
Dobrindt, Ulrich
机构
[1] Univ Wurzburg, Inst Mol Infektiosbiol, D-97070 Wurzburg, Germany
[2] Univ Gottingen, Inst Microbiol & Genet, Gottingen Genom Lab, D-37077 Gottingen, Germany
[3] Univ Pecs, Inst Med Microbiol & Immunol, H-7624 Pecs, Hungary
[4] Biomax Informat AG, D-82152 Martinsried, Germany
[5] Inst Pasteur, Lab Genom Microorganismes Pathogenes, F-75724 Paris 15, France
关键词
fitness; genome comparison; uropathogenic; Escherichia coli;
D O I
10.1073/pnas.0603038103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Uropathogenic Escherichia coli (UPEC) strain 536 (O6:K15:H31) is one of the model organisms of extraintestinal pathogenic E. coli (ExPEC). To analyze this strain's genetic basis of urovirulence, we sequenced the entire genome and compared the data with the genome sequence of UPEC strain CFT073 (O6:K2:H1) and to the available genomes of nonpathogenic E. coli strain MG1655 (K-12) and enterohemorrhagic E. coli. The genome of strain 536 is approximate to 292 kb smaller than that of strain CFT073. Genomic differences between both UPEC are mainly restricted to large pathogenicity islands, parts of which are unique to strain 536 or CFT073. Genome comparison underlines that repeated insertions and deletions in certain parts of the genome contribute to genome evolution. Furthermore, 427 and 432 genes are only present in strain 536 or in both UPEC, respectively. The majority of the latter genes is encoded within smaller horizontally acquired DNA regions scattered all over the genome. Several of these genes are involved in increasing the pathogens' fitness and adaptability. Analysis of virulence-associated traits expressed in the two UPEC 06 strains, together with genome comparison, demonstrate the marked genetic and phenotypic variability among UPEC. The ability to accumulate and express a variety of virulence-associated genes distinguishes ExPEC from many commensals and forms the basis for the individual virulence potential of ExPEC. Accordingly, instead of a common virulence mechanism, different ways exist among ExPEC to cause disease.
引用
收藏
页码:12879 / 12884
页数:6
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